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Ribonucleotides inc...
Ribonucleotides incorporated by the yeast mitochondrial DNA polymerase are not repaired
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- Wanrooij, Paulina H. (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik,Andrei Chabes
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- Engqvist, Martin K M, 1983 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine,Chalmers tekniska högskola,Chalmers University of Technology
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- Forslund, Josefin M. E. (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik,Sjoerd Wanrooij
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- Navarrete, Clara, 1981 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine,University of Gothenburg
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- Nilsson, Anna Karin (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik,Andrei Chabes
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- Sedman, J. (författare)
- Tartu Ülikool,University of Tartu
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- Wanrooij, Sjoerd (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik,Sjoerd Wanrroij
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- Clausen, Anders R, 1979 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology,University of Gothenburg
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- Chabes, Andrei (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik,Molekylär Infektionsmedicin, Sverige (MIMS),Andrei Chabes
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(creator_code:org_t)
- 2017-11-06
- 2017
- Engelska.
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 114:47, s. 12466-12471
- Relaterad länk:
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https://www.pnas.org...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://research.cha...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Incorporation of ribonucleotides into DNA during genome replication is a significant source of genomic instability. The frequency of ribonucleotides in DNA is determined by deoxyribonucleoside triphosphate/ribonucleoside triphosphate (dNTP/rNTP) ratios, by the ability of DNA polymerases to discriminate against ribonucleotides, and by the capacity of repair mechanisms to remove incorporated ribonucleotides. To simultaneously compare how the nuclear and mitochondrial genomes incorporate and remove ribonucleotides, we challenged these processes by changing the balance of cellular dNTPs. Using a collection of yeast strains with altered dNTP pools, we discovered an inverse relationship between the concentration of individual dNTPs and the amount of the corresponding ribonucleotides incorporated in mitochondrial DNA, while in nuclear DNA the ribonucleotide pattern was only altered in the absence of ribonucleotide excision repair. Our analysis uncovers major differences in ribonucleotide repair between the two genomes and provides concrete evidence that yeast mitochondria lack mechanisms for removal of ribonucleotides incorporated by the mtDNA polymerase. Furthermore, as cytosolic dNTP pool imbalances were transmitted equally well into the nucleus and the mitochondria, our results support a view of the cytosolic and mitochondrial dNTP pools in frequent exchange. © 2017, National Academy of Sciences. All rights reserved.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- DNA replication
- DNTP
- Mitochondrial DNA
- Ribonucleotide excision repair
- Ribonucleotide incorporation
- cell nucleus DNA
- deoxyribonucleoside triphosphate
- ribonucleotide
- Article
- biochemical analysis
- excision repair
- mitochondrial genome
- nonhuman
- priority journal
- yeast
- DNTP
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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