Escherichia coli outer membrane vesicles can contribute to sepsis induced cardiac dysfunction.

Svennerholm, Kristina,1981Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för anestesiologi och intensivvård,Institute of Clinical Sciences, Department of Anesthesiology and Intensive care (författare)

Sepsis induced cardiac dysfunction (SIC) is a severe complication to sepsis which significantly worsens patient outcomes. It is known that bacteria have the capacity to release outer membrane vesicles (OMVs), which are nano-sized bilayered vesicles composed of lipids and proteins, that can induce a fatal inflammatory response. The aim of this study was to determine whether OMVs from a uropathogenic Escherichia coli strain can induce cardiac dysfunction, and to elucidate any mechanisms involved. OMVs induced irregular Ca2+ oscillations with a decreased frequency in cardiomyocytes through recordings of intracellular Ca2+ dynamics. Mice were intraperitoneally injected with bacteria-free OMVs, which resulted in increased concentration of pro-inflammatory cytokine levels in blood. Cytokines were increased in heart lysates, and OMVs could be detected in the heart after OMVs injection. Troponin T was significantly increased in blood, and echocardiography showed increased heart wall thickness as well as increased heart rate. This study shows that E. coli OMVs induce cardiac injury in vitro and in vivo, in the absence of bacteria, and may be a causative microbial signal in SIC. The role of OMVs in clinical disease warrant further studies, as bacterial OMVs in addition to live bacteria may be good therapeutic targets to control sepsis.

Park, Kyong-SuGothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xparky (författare)
Wikström, Johannes (författare)
Lässer, Cecilia,1981Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xlasce (författare)
Crescitelli, Rossella,1985Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xcrero (författare)
Shelke, Ganesh V,1986Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xshega (författare)
Jang, Su Chul,1984Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xjangs (författare)
Suzuki, ShintaroGothenburg University,Göteborgs universitet,Krefting Research Centre (författare)
Bandeira, ElgaGothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xbaelg (författare)
Olofsson, Charlotta S,1971Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology(Swepub:gu)xoloch (författare)
Lötvall, Jan,1956Gothenburg University,Göteborgs universitet,Krefting Research Centre(Swepub:gu)xlotja (författare)
Göteborgs universitetInstitutionen för kliniska vetenskaper, Avdelningen för anestesiologi och intensivvård (creator_code:org_t)

Av författaren/redakt...: Svennerholm, Kri ...; Park, Kyong-Su; Wikström, Johann ...; Lässer, Cecilia, ...; Crescitelli, Ros ...; Shelke, Ganesh V ...; visa fler...; Jang, Su Chul, 1 ...; Suzuki, Shintaro; Bandeira, Elga; Olofsson, Charlo ...; Lötvall, Jan, 19 ...; visa färre...

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