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Electrochemical Inv...
Electrochemical Investigation of the Interaction between Catecholamines and ATP
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- Taleat, Zahra, 1982 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Estevez-Herrera, J. (author)
- Universidad de la Laguna,University of La Laguna
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- Dunevall, Johan, 1984 (author)
- Universidad de la Laguna,University of La Laguna,Chalmers tekniska högskola,Chalmers University of Technology
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Dunevall, J. (author)
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- Ewing, Andrew G, 1957 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg,Chalmers tekniska högskola,Chalmers University of Technology
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- Borges, R. (author)
- Universidad de la Laguna,University of La Laguna
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(creator_code:org_t)
- 2018-01-22
- 2018
- English.
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In: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 90:3, s. 1601-1607
- Related links:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The study of the colligative properties of adenosine 5'-triphosphate (ATP) and catecholamines has received the attention of scientists for decades, as they could explain the capabilities of secretory vesicles (SVs) to accumulate neurotransmitters. In this Article, we have applied electrochemical methods to detect such interactions in vitro, at the acidic pH of SVs (pH 5.5) and examined the effect of compounds having structural similarities that correlate with functional groups of ATP (adenosine, phosphoric acid and sodium phosphate salts) and catecholamines (catechol). Chronoamperometry and fast scan cyclic voltammetry (FSCV) provide evidence compatible with an interaction of the catechol and adenine rings. This interaction is also reinforced by an electrostatic interaction between the phosphate group of ATP and the protonated ammonium group of catecholamines. Furthermore, chronoamperometry data suggest that the presence of ATP subtlety reduces the apparent diffusion coefficient of epinephrine in aqueous media that adds an additional factor leading to a slower rate of catecholamine exocytosis. This adds another plausible mechanism to regulate individual exocytosis events to alter communication.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Keyword
- adrenal chromaffin granules
- nuclear-magnetic-resonance
- single-cell
- amperometry
- divalent metal-ions
- diffusion-coefficients
- chromogranin-a
- exocytosis
- fusion
- vesicles
- release
- Chemistry
- emical microenvironment
Publication and Content Type
- ref (subject category)
- art (subject category)
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