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Pentacyclic adenine...
Pentacyclic adenine: a versatile and exceptionally bright fluorescent DNA base analogue
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- Bood, Mattias (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg
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- Füchtbauer, Anders Foller, 1984 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Sandberg Wranne, Moa, 1986 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Ro, J. J. (author)
- Pohang University of Science and Technology
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- Sarangamath, Sangamesh, 1992 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,University of Edinburgh
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- El-Sagheer, A. H. (author)
- Suez University
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- Rupert, Deborah, 1986 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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Fisher, R. S. (author)
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- Magennis, S. W. (author)
- University of Glasgow
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- Jones, A. C. (author)
- University of Edinburgh
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- Höök, Fredrik, 1966 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Brown, T. (author)
- University Of Oxford
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- Kim, B. H. (author)
- Pohang University of Science and Technology
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- Dahlen, A. (author)
- AstraZeneca AB
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- Wilhelmsson, Marcus, 1974 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Grötli, Morten (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg
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(creator_code:org_t)
- 2018
- 2018
- English.
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In: Chemical Science. - : Royal Society of Chemistry (RSC). - 2041-6520 .- 2041-6539. ; 9:14, s. 3494-3502
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Abstract
Subject headings
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- Emissive base analogs are powerful tools for probing nucleic acids at the molecular level. Herein we describe the development and thorough characterization of pentacyclic adenine (pA), a versatile base analog with exceptional fluorescence properties. When incorporated into DNA, pA pairs selectively with thymine without perturbing the B-form structure and is among the brightest nucleobase analogs reported so far. Together with the recently established base analog acceptor qAnitro, pA allows accurate distance and orientation determination via Forster resonance energy transfer (FRET) measurements. The high brightness at emission wavelengths above 400 nm also makes it suitable for fluorescence microscopy, as demonstrated by imaging of single liposomal constructs coated with cholesterolanchored pA-dsDNA, using total internal reflection fluorescence microscopy. Finally, pA is also highly promising for two-photon excitation at 780 nm, with a brightness (5.3 GM) that is unprecedented for a base analog.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
- NATURVETENSKAP -- Kemi -- Teoretisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Theoretical Chemistry (hsv//eng)
Keyword
- pteridine nucleoside analogs
- emissive rna alphabet
- 2-photon
- excitation
- nucleic-acid
- quadracyclic adenine
- nucleobase analogs
- tc
- 2-aminopurine
- nucleotides
- environment
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Bood, Mattias
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Füchtbauer, Ande ...
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Sandberg Wranne, ...
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Ro, J. J.
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Sarangamath, San ...
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El-Sagheer, A. H ...
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show more...
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Rupert, Deborah, ...
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Fisher, R. S.
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Magennis, S. W.
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Jones, A. C.
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Höök, Fredrik, 1 ...
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Brown, T.
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Kim, B. H.
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Dahlen, A.
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Wilhelmsson, Mar ...
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Grötli, Morten
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Atom and Molecul ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Other Physics To ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Theoretical Chem ...
- Articles in the publication
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Chemical Science
- By the university
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University of Gothenburg
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Chalmers University of Technology