Sökning: id:"swepub:oai:gup.ub.gu.se/268994" >
Long-Term Stress Di...
Long-Term Stress Disrupts the Structural and Functional Integrity of GABAergic Neuronal Networks in the Medial Prefrontal Cortex of Rats
- Artikel/kapitelEngelska2018
Förlag, utgivningsår, omfång ...
-
2018-06-20
-
Frontiers Media SA,2018
Nummerbeteckningar
-
LIBRIS-ID:oai:gup.ub.gu.se/268994
-
https://gup.ub.gu.se/publication/268994URI
-
https://doi.org/10.3389/fncel.2018.00148DOI
Kompletterande språkuppgifter
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
Clinical and experimental data suggest that fronto-cortical GABAergic deficits contribute to the pathophysiology of major depressive disorder (MDD). To further test this hypothesis, we used a well characterized rat model for depression and examined the effect of stress on GABAergic neuron numbers and GABA-mediated synaptic transmission in the medial prefrontal cortex (mPFC) of rats. Adult male Wistar rats were subjected to 9-weeks of chronic mild stress (CMS) and based on their hedonic-anhedonic behavior they were behaviorally phenotyped as being stress-susceptible (anhedonic) or stress-resilient. Post mortem quantitative histopathology was used to examine the effect of stress on parvalbumin (PV)-, calretinin- (CR), calbindin- (CB), cholecystokinin- (CCK), somatostatin-(SST) and neuropeptide Y-positive (NPY+) GABAergic neuron numbers in all cortical subareas of the mPFC (anterior cingulate (Cg1), prelimbic (PrL) and infralimbic (IL) cortexes). In vitro, whole-cell patch-clamp recordings from layer II-III pyramidal neurons of the ventral mPFC was used to examine GABAergic neurotransmission. The cognitive performance of the animals was assessed in a hippocampal-prefrontal-cortical circuit dependent learning task. Stress exposure reduced the number of CCK-, CR- and PV-positive GABAergic neurons in the mPFC, most prominently in the IL cortex. Interestingly, in the stress-resilient animals, we found higher number of neuropeptide Y-positive neurons in the entire mPFC. The electrophysiological analysis revealed reduced frequencies of spontaneous and miniature IPSCs in the anhedonic rats and decreased release probability of perisomatic-targeting GABAergic synapses and alterations in GABA(B) receptor mediated signaling. In turn, pyramidal neurons showed higher excitability. Anhedonic rats were also significantly impaired in the object-place paired-associate learning task. These data demonstrate that long-term stress results in functional and structural deficits of prefrontal GABAergic networks. Our findings support the concept that fronto-limbic GABAergic dysfunctions may contribute to emotional and cognitive symptoms of MDD.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Vardya, I.
(författare)
-
Varga, Z.
(författare)
-
Febbraro, F.
(författare)
-
Csabai, D.
(författare)
-
Martis, L. S.
(författare)
-
Hoigaard, K.
(författare)
-
Henningsen, K.
(författare)
-
Bouzinova, E. V.
(författare)
-
Miseta, A.
(författare)
-
Jensen, KimmoGothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology(Swepub:gu)xjenki
(författare)
-
Wiborg, O.
(författare)
-
Göteborgs universitetInstitutionen för neurovetenskap och fysiologi
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Frontiers in Cellular Neuroscience: Frontiers Media SA121662-5102
Internetlänk
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Czeh, B.
-
Vardya, I.
-
Varga, Z.
-
Febbraro, F.
-
Csabai, D.
-
Martis, L. S.
-
visa fler...
-
Hoigaard, K.
-
Henningsen, K.
-
Bouzinova, E. V.
-
Miseta, A.
-
Jensen, Kimmo
-
Wiborg, O.
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Neurologi
- Artiklar i publikationen
-
Frontiers in Cel ...
- Av lärosätet
-
Göteborgs universitet