Mass Spectrometry Imaging Shows Cocaine and Methylphenidate Have Opposite Effects on Major Lipids in Drosophila Brain

Philipsen, Mai H.,1988Gothenburg University,Göteborgs universitet,National Center for Imaging Mass Spectrometry,Chalmers tekniska högskola,Chalmers University of Technology (författare)

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used to study the effects of cocaine versus methylphenidate administration on both the localization and abundance of lipids in Drosophila melanogaster brain. A J105 ToF-SIMS with a 40 keV gas cluster primary ion source enabled us to probe molecular ions of biomolecules on the fly with a spatial resolution of similar to 3 mu m, giving us unique insights into the effect of these drugs on molecular lipids in the nervous system. Significant changes in phospholipid composition were observed in the central brain for both. Principal components image analysis revealed that changes occurred mainly for phosphatidylcholines, phosphatidylethanolamines, and phosphatidylinositols. When the lipid changes caused by cocaine were compared with those induced by methylphenidate, it was shown that these drugs exert opposite effects on the brain lipid structure. We speculate that this might relate to the molecular mechanism of cognition and memory.

Phan, Nhu TN,1981Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,National Center for Imaging Mass Spectrometry,Department of Chemistry and Molecular Biology,Universitätsmedizin Göttingen,University Medical Center Göttingen,University of Gothenburg(Swepub:gu)xphant (författare)
Fletcher, John S.Gothenburg University,Göteborgs universitet,National Center for Imaging Mass Spectrometry,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg(Swepub:cth)fletcher (författare)
Malmberg, Per,1974Gothenburg University,Göteborgs universitet,National Center for Imaging Mass Spectrometry,Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)malmper (författare)
Ewing, Andrew G,1957Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,National Center for Imaging Mass Spectrometry,Department of Chemistry and Molecular Biology,University of Gothenburg,Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)andrewe (författare)
Göteborgs universitetNational Center for Imaging Mass Spectrometry (creator_code:org_t)

Ingår i:Acs Chemical Neuroscience: American Chemical Society (ACS)9:6, s. 1462-14681948-7193

Av författaren/redakt...: Philipsen, Mai H ...; Phan, Nhu TN, 19 ...; Fletcher, John S ...; Malmberg, Per, 1 ...; Ewing, Andrew G, ...

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