Exploring plasmonic coupling as a stimuli responsive contrast mechanism in multiphoton microscopy

• A novel approach for optical biosensing can be obtained based multiphoton induced luminescence (MIL) and its dependence on plasmonic coupling. It has been shown that the proximity of spherical AuNPs determines the generation of MIL in far-field multiphoton laser scanning microscopy (MPM). A stimuli responsive contrast mediator with high sensitivity can be created by controlling the aggregated state of AuNP. In this study we explore a system based on spherical AuNPs functionalized with beta-cyclodextrin and multiple beta-D-lactose units (lacto-CD-AuNP). The aim of the beta-D- lactose units is to target cancer cells, based on overexpression of galectin3 (Gal-3) receptors. The results demonstrate that clustering of particles, and thereby MIL signal, was only acquired from tumor cell lines, i.e., SK-MEL-28 and A431, while not from normal keratinocytes (HEKn). Thus further studies should be undertaken to translate the concept to a preclinical setting.

Subject headings

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Cancer och onkologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Cancer and Oncology (hsv//eng)

Keyword

Gold nanoparticles

multiphoton microscopy

biosensing

galactin-3

skin cancer

keratinocytes

gold nanoparticles

Gold nanoparticles; multiphoton microscopy; biosensing; galactin-3; skin cancer; keratinocytes; multiphoton luminescence

Publication and Content Type

ref (subject category)

kon (subject category)