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Artificial spider s...
Artificial spider silk supports and guides neurite extension in vitro
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- Hansson, Magnus L. (author)
- Karolinska Inst, Dept Biosci & Nutr, S-14186 Huddinge, Sweden.;Karolinska Inst, Dept Neurosci, Expt Traumatol Unit, Biomed B8, S-17177 Stockholm, Sweden.
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- Chatterjee, Urmimala (author)
- Karolinska Institutet
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- Francis, Juanita (author)
- Karolinska Inst, Dept Biosci & Nutr, S-14186 Huddinge, Sweden.
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- Arndt, Tina (author)
- Karolinska Institutet
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- Broman, Christian (author)
- Karolinska Inst, Dept Neurosci, Expt Traumatol Unit, Biomed B8, S-17177 Stockholm, Sweden.
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- Johansson, Jan (author)
- Karolinska Institutet
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- Sköld, Mattias K. (author)
- Karolinska Institutet,Uppsala universitet,Enblad: Neurokirurgi,Karolinska Inst, Dept Neurosci, Expt Traumatol Unit, Biomed B8, S-17177 Stockholm, Sweden.
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- Rising, Anna (author)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Karolinska Institutet,Institutionen för anatomi, fysiologi och biokemi,Department of Anatomy, Physiology and Biochemistry (AFB),Karolinska Institute
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Karolinska Institutet Karolinska Inst, Dept Biosci & Nutr, S-14186 Huddinge, Sweden;Karolinska Inst, Dept Neurosci, Expt Traumatol Unit, Biomed B8, S-17177 Stockholm, Sweden. (creator_code:org_t)
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- John Wiley & Sons, 2021
- 2021
- English.
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In: The FASEB Journal. - : John Wiley & Sons. - 0892-6638 .- 1530-6860. ; 35:11
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Abstract
Subject headings
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- Surgical intervention with the use of autografts is considered the gold standard to treat peripheral nerve injuries. However, a biomaterial that supports and guides nerve growth would be an attractive alternative to overcome problems with limited availability, morbidity at the site of harvest, and nerve mismatches related to autografts. Native spider silk is a promising material for construction of nerve guidance conduit (NGC), as it enables regeneration of cm-long nerve injuries in sheep, but regulatory requirements for medical devices demand synthetic materials. Here, we use a recombinant spider silk protein (NT2RepCT) and a functionalized variant carrying a peptide derived from vitronectin (VN-NT2RepCT) as substrates for nerve growth support and neurite extension, using a dorsal root ganglion cell line, ND7/23. Two-dimensional coatings were benchmarked against poly-d-lysine and recombinant laminins. Both spider silk coatings performed as the control substrates with regards to proliferation, survival, and neurite growth. Furthermore, NT2RepCT and VN-NT2RepCT spun into continuous fibers in a biomimetic spinning set-up support cell survival, neurite growth, and guidance to an even larger extent than native spider silk. Thus, artificial spider silk is a promising biomaterial for development of NGCs.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomaterialvetenskap (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomaterials Science (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Medicinteknik -- Medicinsk material- och protesteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Medical Engineering -- Medical Materials (hsv//eng)
Keyword
- biomaterial
- nerve guidance conduit
- peripheral nerve
- recombinant spider silk
Publication and Content Type
- ref (subject category)
- art (subject category)
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