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Novel ELISA for thr...
Novel ELISA for thrombospondin type 1 domain-containing 7A autoantibodies in membranous nephropathy
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Zaghrini, C. (author)
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Seitz-Polski, B. (author)
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Justino, J. (author)
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Dolla, G. (author)
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Payre, C. (author)
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Jourde-Chiche, N. (author)
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Van de Logt, A. E. (author)
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Booth, C. (author)
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Rigby, E. (author)
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- Lönnbro-Widgren, Jennie (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine
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- Nyström, Jenny, 1972 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin,Institute of Medicine
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Mariat, C. (author)
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Cui, Z. (author)
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Wetzels, J. F. M. (author)
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Ghiggeri, G. (author)
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Beck, L. H. (author)
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Ronco, P. (author)
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Debiec, H. (author)
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Lambeau, G. (author)
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(creator_code:org_t)
- Elsevier BV, 2019
- 2019
- English.
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In: Kidney International. - : Elsevier BV. - 0085-2538. ; 95:3, s. 666-679
- Related links:
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https://www.hal.inse...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Autoantibodies against phospholipase A2 receptor 1 (PLA2R1) and thrombospondin type 1 domain-containing 7A (THSD7A) are emerging as biomarkers to classify membranous nephropathy (MN) and to predict outcome or response to treatment. Anti-THSD7A autoantibodies are detected by Western blot and indirect immunofluorescence test (IIFT). Here, we developed a sensitive enzyme-linked immunosorbent assay (ELISA) optimized for quantitative detection of anti-THSD7A autoantibodies. Among 1012 biopsy-proven MN patients from 6 cohorts, 28 THSD7A-positive patients were identified by ELISA, indicating a prevalence of 2.8%. By screening additional patients, mostly referred because of PLA2R1-unrelated MN, we identified 21 more cases, establishing a cohort of 49 THSD7A-positive patients. Twenty-eight patients (57%) were male, and male patients were older than female patients (67 versus 49 years). Eight patients had a history of malignancy, but only 3 were diagnosed with malignancy within 2 years of MN diagnosis. We compared the results of ELISA, IIFT, Western blot, and biopsy staining, and found a significant correlation between ELISA and IIFT titers. Anti-THSD7A autoantibodies were predominantly IgG4 in all patients. Eight patients were double positive for THSD7A and PLA2R1. Levels of anti-THSD7A autoantibodies correlated with disease activity and with response to treatment. Patients with high titer at baseline had poor clinical outcome. In a subgroup of patients with serial titers, persistently elevated anti-THSD7A autoantibodies were observed in patients who did not respond to treatment or did not achieve remission. We conclude that the novel anti-THSD7A ELISA can be used to identify patients with THSD7A-associated MN and to monitor autoantibody titers during treatment.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Urologi och njurmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Urology and Nephrology (hsv//eng)
Keyword
- clinical outcome
- ELISA
- malignancy
- membranous nephropathy
- sex
- THSD7A
- phospholipase a(2) receptor
- anti-pla(2)r antibodies
- pla2r
- autoantibodies
- glomerular-diseases
- pregnancy
- rituximab
- subclass
- deposits
- outcomes
- cancer
- Urology & Nephrology
- hieppati a
- 1993
- new england journal of medicine
- v329
- p85
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Zaghrini, C.
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Seitz-Polski, B.
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Justino, J.
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Dolla, G.
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Payre, C.
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Jourde-Chiche, N ...
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show more...
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Van de Logt, A. ...
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Booth, C.
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Rigby, E.
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Lönnbro-Widgren, ...
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Nyström, Jenny, ...
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Mariat, C.
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Cui, Z.
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Wetzels, J. F. M ...
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Ghiggeri, G.
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Beck, L. H.
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Ronco, P.
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Debiec, H.
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Lambeau, G.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Urology and Neph ...
- Articles in the publication
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Kidney Internati ...
- By the university
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University of Gothenburg