WFRF:(Nestor David 1992 )
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- Nestor, David,1992-Department of Laboratory Medicine, Clinical Microbiology, University Hospital, Örebro, Sweden (author)
Evaluation of the FilmArray (TM) Meningitis/Encephalitis panel with focus on bacteria and Cryptococcus spp
- Article/chapterEnglish2019
Publisher, publication year, extent ...
- Elsevier BV,2019
Numbers
- LIBRIS-ID:oai:gup.ub.gu.se/278818
- https://gup.ub.gu.se/publication/278818URI
- https://doi.org/10.1016/j.mimet.2019.01.003DOI
- https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-109732URI
Supplementary language notes
- Language:English
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- SwePubSwePub
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Notes
- Purpose: Molecular methods provide fast and accurate detection of both bacteria and viruses in the cerebrospinal fluid (CSF) causing infection in the central nervous system (CNS). In the present study we evaluated the bacterial detection performance of the fully automated FilmArray (TM) Meningitis/Encephalitis (ME) panel (bioMerieux) by comparing it with culture and multiplexed in-house PCR. Methods: Three sample types were analysed; Contrived samples with known bacterial/fungal concentration (n = 29), clinical samples from patients with verified cause of CNS infection (n = 17) and external quality assessment (EQA) samples (n = 11). Another six samples were purposely prepared with multiple targets to evaluate multiplex capacity. Results: The FilmArray (TM) had a slightly higher limit of detection for Streptococcus pneumoniae, Neisseria meningitidis, Listeria monocytogenes and Streptococcus agalactiae compared to in-house PCR methods but performed equal or better when compared to culture. The FilmArray (TM) ME panel detected the expected pathogen in 17 of 17 clinical samples and yielded detection of three additional viruses of which one was confirmed with comparator techniques. All but one of the EQA samples were correctly detected. Conclusions: The results of this study are promising and the FilmArray (TM) ME panel could add to the diagnostic algorithm in CNS-infections. However, the limit of detection for the important pathogens N. meningitidis and S. pneumoniae could be improved.
Subject headings and genre
- MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Mikrobiologi inom det medicinska området hsv//swe
- MEDICAL AND HEALTH SCIENCES Basic Medicine Microbiology in the medical area hsv//eng
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Klinisk laboratoriemedicin hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Clinical Laboratory Medicine hsv//eng
- Multiplex PCR
- CSF analysis
- CNS infections
- Rapid diagnostics
- cerebrospinal-fluid
- pcr
- diagnosis
- meningitis
- DNA
- CNS infections
Added entries (persons, corporate bodies, meetings, titles ...)
- Skovbjerg, Susann,1973Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xjsusm (author)
- Lignell, M.Department of Bacteriology, Clinical Microbiological Laboratory, Sahlgrenska University Hospital, Gothenburg, Sweden (author)
- Skovbjerg, Susann,1973Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xjsusm (author)
- Mölling, Paula,1971-Region Örebro län,Örebro universitet, Institutionen för medicinska vetenskaper,Department of Laboratory Medicine, Clinical Microbiology(Swepub:oru)pamn (author)
- Sundqvist, Martin,1974-Region Örebro län,Örebro universitet, Institutionen för medicinska vetenskaper,Department of Laboratory Medicine, Clinical Microbiology(Swepub:oru)mist (author)
- Department of Laboratory Medicine, Clinical Microbiology, University Hospital, Örebro, SwedenInstitutionen för biomedicin, avdelningen för infektionssjukdomar (creator_code:org_t)
Related titles
- In:Journal of Microbiological Methods: Elsevier BV157, s. 113-1160167-70121872-8359
Internet link
- https://doi.org/10.1016/j.mimet.2019.01.003
- https://doi.org/10.1016/j.mimet.2019.01.003Fulltext
- https://oru.diva-portal.org/smash/get/diva2:1812070/FULLTEXT01.pdffulltext:print
- https://gup.ub.gu.se/publication/278818
- https://doi.org/10.1016/j.mimet.2019.01.003
- https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-109732
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