Determination of mechanical and rheological properties of a cell-loaded peptide gel during ECM production

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Barreto Henriksson, HelenaSahlgrenska universitetssjukhuset,Sahlgrenska University Hospital,Sahlgrenska Hospital, Sweden (author)

Determination of mechanical and rheological properties of a cell-loaded peptide gel during ECM production

Article/chapterEnglish2019

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Elsevier BV,2019

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LIBRIS-ID:oai:gup.ub.gu.se/280871
https://gup.ub.gu.se/publication/280871URI
https://doi.org/10.1016/j.ijpharm.2019.04.028DOI
https://research.chalmers.se/publication/510025URI
https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-38672URI

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Language:English

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SwePubSwePub

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Funding details: Vetenskapsrådet; Funding details: Västra Götalandsregionen; Funding details: IngaBritt och Arne Lundbergs Forskningsstiftelse; Funding text 1: The financial contributions of the VINN Excellence center SuMo Biomaterials, VINNMER, the Swedish Research Council, ALF Västra Götaland, Inga-Britt and Arne Lundberg Foundation, the Chalmers Area of Advance Materials and the Dr. Felix Neuberghs Foundation are gratefully acknowledged.
The development of an injectable biomaterial that supports cell survival and maintains or promotes nucleus pulposus (NP) phenotype could aid delivery of cells to degenerated NPs causing low back pain. Mesenchymal cells were loaded and grown in a synthetic peptide gel, PuraMatrix (R). Cells were observed within the gels over 0-28 days, and accumulation of glycosaminoglycans were detected by histological staining. The mechanical properties of the cell-loaded constructs, and the change of the mechanical properties were studied using stress relaxation of the gels under compression and confinement. The PuraMatrix (R) gel was shown to relax fast on compression indicating that the fluid could easily flow out of the gel, and thus indicating the presence of large pores/voids. The presence of these pores/voids was further supported by high mobility of dextran molecules, determined using fluorescence recovery after photo bleaching. The stress required to deform the cell-loaded constructs to a specific strain increases at day 21, at which point the presence of glycosaminoglycans within the cell-loaded constructs was also observed. The results provide evidence of changes in mechanical properties of the PuraMatrix (R) matrix upon excretion of the extracellular matrix by the cells.

Subject headings and genre

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Farmaceutiska vetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Pharmaceutical Sciences hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Medicinsk bioteknologi hsv//swe
MEDICAL AND HEALTH SCIENCES Medical Biotechnology Medical Biotechnology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinsk bioteknologi Biomaterialvetenskap hsv//swe
MEDICAL AND HEALTH SCIENCES Medical Biotechnology Biomaterials Science hsv//eng
Extracellular matrix
PuraMatrix
hMSc
Stress relaxation
Glycosaminoglycans
GAG
in-vitro chondrogenesis
mesenchymal stem-cells
human nucleus pulposus
low-back-pain
intervertebral disc
gene-expression
hyaluronic-acid
degeneration
hydrogels
scaffold
PuraMatrix

Added entries (persons, corporate bodies, meetings, titles ...)

Llorente, M.Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden (author)
Larsson, Anette,1966Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden(Swepub:cth)anettel (author)
Brisby, Helena,1965Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för ortopedi,Institute of Clinical Sciences, Department of Orthopaedics,University of Gothenburg,University of Gothenburg, Sweden(Swepub:gu)xbrish (author)
Gold, Julie,1963Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden(Swepub:cth)f7xjg (author)
Schuster, E.RISE,Jordbruk och livsmedel,Chalmers University of Technology, Sweden (author)
Ström, Anna,1976Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden(Swepub:cth)annastr (author)
Sahlgrenska universitetssjukhusetSahlgrenska Hospital, Sweden (creator_code:org_t)

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In:International Journal of Pharmaceutics: Elsevier BV563, s. 437-4440378-51731873-3476

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MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Pharmaceutical S ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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