In Vivo Human Cartilage Formation in Three-Dimensional Bioprinted Constructs with a Novel Bacterial Nanocellulose Bioink

Apelgren, PeterGothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery,Sahlgrenska universitetssjukhuset,Sahlgrenska University Hospital (author)

Bacterial nanocellulose (BNC) is a 3D network of nanofibrils exhibiting excellent biocompatibility. Here, we present the aqueous counter collision (ACC) method of BNC disassembly to create bioink with suitable properties for cartilage-specific 3D-bioprinting. BNC was disentangled by ACC, and fibril characteristics were analyzed. Bioink printing fidelity and shear-thinning properties were evaluated. Cell-laden bioprinted grid constructs (5 X 5 X 1 mm(3)) containing human nasal chondrocytes (10 M mL(-1)) were implanted in nude mice and explanted after 30 and 60 days. Both ACC and hydrolysis resulted in significantly reduced fiber lengths, with ACC resulting in longer fibrils and fewer negative charges relative to hydrolysis. Moreover, ACC-BNC bioink showed outstanding printability, postprinting mechanical stability, and structural integrity. In vivo, cell-laden structures were rapidly integrated, maintained structural integrity, and showed chondrocyte proliferation, with 32.8 +/- 13.8 cells per mm(2) observed after 30 days and 85.6 +/- 30.0 cells per mm(2) at day 60 (p = 0.002). Furthermore, a full-thickness skin graft was attached and integrated completely on top of the 3D-bioprinted construct. The novel ACC disentanglement technique makes BNC biomaterial highly suitable for 3D-bioprinting and clinical translation, suggesting cell-laden 3D-bioprinted ACC-BNC as a promising solution for cartilage repair.

Karabulut, Erdem,1983Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)erdemk (author)
Amoroso, Matteo,1984Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery,Sahlgrenska universitetssjukhuset,Sahlgrenska University Hospital(Swepub:gu)xamoma (author)
Mantas, Athanasios,1984(Swepub:cth)mantas (author)
Martinez Avila, Hector,1985(Swepub:cth)hectorm (author)
Kölby, Lars,1963Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi,Institute of Clinical Sciences, Department of Plastic Surgery,Sahlgrenska universitetssjukhuset,Sahlgrenska University Hospital(Swepub:gu)xkolbl (author)
Kondo, T.Kyushu University (author)
Toriz Gonzalez, Guillermo,1968Chalmers tekniska högskola,Chalmers University of Technology,Universidad de Guadalajara,University of Guadalajara(Swepub:cth)guillert (author)
Gatenholm, Paul,1956Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)gatenh (author)
Göteborgs universitetInstitutionen för kliniska vetenskaper, Avdelningen för plastikkirurgi (creator_code:org_t)

In:Acs Biomaterials Science & Engineering: American Chemical Society (ACS)5:5, s. 2482-24902373-9878

By the author/editor: Apelgren, Peter; Karabulut, Erdem ...; Amoroso, Matteo, ...; Mantas, Athanasi ...; Martinez Avila, ...; Kölby, Lars, 196 ...; show more...; Kondo, T.; Toriz Gonzalez, ...; Gatenholm, Paul, ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Biomaterials Sci ...

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Industrial Biote ...; and Bio Materials

ENGINEERING AND TECHNOLOGY: ENGINEERING AND ...; and Industrial Biote ...; and Bioengineering E ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Medical Biotechn ...; and Medical Biotechn ...

By the university: University of Gothenburg; Chalmers University of Technology

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