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DNA analysis of low...
DNA analysis of low- and high-density fractions defines heterogeneous subpopulations of small extracellular vesicles based on their DNA cargo and topology
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- Lázaro-Ibáñez, Elisa (författare)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre
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- Lässer, Cecilia, 1981 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Krefting Research Centre,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
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- Shelke, Ganesh V, 1986 (författare)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre
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- Crescitelli, Rossella, 1985 (författare)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre
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- Jang, Su Chul, 1984 (författare)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre
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- Cvjetkovic, Aleksander (författare)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre
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- García-Rodríguez, Anaís (författare)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre
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- Lötvall, Jan, 1956 (författare)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre
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(creator_code:org_t)
- 2019-08-27
- 2019
- Engelska.
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Ingår i: Journal of Extracellular Vesicles. - : Wiley. - 2001-3078. ; 8:1
- Relaterad länk:
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https://www.tandfonl...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Extracellular vesicles have the capacity to transfer lipids, proteins, and nucleic acids between cells, thereby influencing the recipient cell's phenotype. While the role of RNAs in EVs has been extensively studied, the function of DNA remains elusive. Here, we distinguished novel heterogeneous subpopulations of small extracellular vesicles (sEVs) based on their DNA content and topology. Low- and high-density sEV subsets from a human mast cell line (HMC-1) and an erythroleukemic cell line (TF-1) were separated using high-resolution iodixanol density gradients to discriminate the nature of the DNA cargo of the sEVs. Paired comparisons of the sEV-associated DNA and RNA molecules showed that RNA was more abundant than DNA and that most of the DNA was present in the high-density fractions, demonstrating that sEV subpopulations have different DNA content. DNA was predominately localised on the outside or surface of sEVs, with only a small portion being protected from enzymatic degradation. Whole-genome sequencing identified DNA fragments spanning all chromosomes and mitochondrial DNA when sEVs were analysed in bulk. Our work contributes to the understanding of how DNA is associated with sEVs and thus provides direction for distinguishing subtypes of EVs based on their DNA cargo and topology.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Small extracellular vesicles
- sEVs
- exosomes
- extracellular DNA
- cell-free DNA
- DNA topology
- histones
- density gradient
- double-stranded DNA
- horizontal transfer
- mediated transfer
- mutant
- kras
- exosomes
- microvesicles
- activate
- cells
- mechanism
- program
- Cell Biology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
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Lázaro-Ibáñez, E ...
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Lässer, Cecilia, ...
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Shelke, Ganesh V ...
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Crescitelli, Ros ...
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Jang, Su Chul, 1 ...
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Cvjetkovic, Alek ...
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García-Rodríguez ...
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Lötvall, Jan, 19 ...
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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och Cell och molekyl ...
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Journal of Extra ...
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Göteborgs universitet