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  • Willemse, E. A. J. (author)

Pre-analytical stability of novel cerebrospinal fluid biomarkers

  • Article/chapterEnglish2019

Publisher, publication year, extent ...

  • Elsevier BV,2019

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/284227
  • https://gup.ub.gu.se/publication/284227URI
  • https://doi.org/10.1016/j.cca.2019.07.024DOI

Supplementary language notes

  • Language:English

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  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Stability of the cerebrospinal fluid (CSF) composition under different pre-analytical conditions is relevant for the diagnostic potential of biomarkers. Our aim was to examine the pre-analytical stability of promising CSF biomarkers that are currently evaluated for their discriminative use in various neurological diseases. Pooled CSF was aliquoted and experimentally exposed to delayed storage: 0, 1, 2, 4, 24, 72, or 168 h at 4 °C or room temperature (RT), or 1–4 months at −20 °C; or up to 7 freeze/thaw (f/t) cycles, before final storage at −80 °C. Eleven CSF biomarkers were screened using immunoassays, liquid chromatography, or enzymatic methods. Levels of neurogranin (truncP75), chitinase-3-like protein (YKL-40), beta-site amyloid precursor protein cleaving enzyme 1 (BACE1), acetylcholinesterase (AChE) enzymatic activity, theobromine, secreted protein acidic and rich in cysteine-like 1 (SPARCL-1) and homovanillic acid (HVA) levels were not affected by the applied storage conditions. 3-Methoxy-4-hydroxyphenylglycol (MHPG) levels linearly and strongly decreased after 4 h at RT (−10%) or 24 h at 4 °C (−27%), and with 6% after every f/t cycle. 5-Methyltetrahydrofolate (5-MTHF) (−29% after 1 week at RT) and 5-hydroxyindoleacetic acid levels (5-HIAA) (−16% after 1 week at RT) were reduced and 3,4-dihydroxyphenylacetic acid (DOPAC) levels (+22% after 1 week at RT) increased, but only after >24 h at RT. Ten out of eleven potential CSF novel biomarkers showed very limited change under common storage and f/t conditions, suggesting that these CSF biomarkers can be trustfully tested under the pre-analytical conditions present across different cohorts. © 2019 Elsevier B.V.

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Added entries (persons, corporate bodies, meetings, titles ...)

  • Vermeiren, Y. (author)
  • Garcia-Ayllon, M. S. (author)
  • Bridel, C. (author)
  • De Deyn, P. P. (author)
  • Engelborghs, S. (author)
  • van der Flier, W. M. (author)
  • Jansen, E. E. W. (author)
  • Lopez-Font, I. B. (author)
  • Mendes, V. (author)
  • Manadas, B. (author)
  • de Roeck, N. (author)
  • Saez-Valero, J. (author)
  • Struys, E. A. (author)
  • Vanmechelen, E. (author)
  • Andreasson, Ulf,1968Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology(Swepub:gu)xaulfz (author)
  • Teunissen, C. E. (author)
  • Göteborgs universitetInstitutionen för neurovetenskap och fysiologi (creator_code:org_t)

Related titles

  • In:Clinica Chimica Acta: Elsevier BV497, s. 204-2110009-8981

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