Vesicular Transmitter Content in Chromaffin Cells Can Be Regulated via Extracellular ATP

Larsson, AnnaGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology (författare)

The energy carrying molecule adenosine triphosphate (ATP) has been implicated for its role in modulation of chemical signaling for some time. Despite this, the precise effects and mechanisms of action of ATP on secretory cells are not well-known. Here, bovine chromaffin cells have been used as a model system to study the effects of extracellular ATP in combination with the catecholamine transmitter norepinephrine (NE). Both transmitter storage and exocytotic release were quantified using complementary amperometric techniques. Although incubation with NE alone did not cause any changes to either transmitter storage or release, coincubation with NE and ATP resulted in a significant increase that was concentration dependent. To probe the potential mechanisms of action, a slowly hydrolyzable version of ATP, ATP-gamma-S, was used either alone or together with NE. The result implicates two different behaviors of ATP acting on both the purinergic autoreceptors and as a source of the energy needed to load chromaffin cell vesicles.

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
ATP
amperometry
intracellular vesicle impact cytometry
exocytosis
vesicle content
catecholamine uptake
quantal size
exocytosis
chromogranins
storage
vesicles
release
norepinephrine
accumulation
pharmacology
Biochemistry & Molecular Biology
Pharmacology & Pharmacy
Neurosciences
& Neurology

Majdi, Soodabeh,1980Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xmajso (författare)
Borges, R. (författare)
Ewing, Andrew G,1957Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xewian (författare)
Göteborgs universitetInstitutionen för kemi och molekylärbiologi (creator_code:org_t)

Ingår i:ACS Chemical Neuroscience: American Chemical Society (ACS)10:11, s. 4735-47401948-7193

Av författaren/redakt...: Larsson, Anna; Majdi, Soodabeh, ...; Borges, R.; Ewing, Andrew G, ...

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