Effect of Kinase Inhibiting RNase Attenuator (KIRA) Compounds on the Formation of Face-to-Face Dimers of Inositol-Requiring Enzyme 1: Insights from Computational Modeling

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Carlesso, Antonio,1990Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology (författare)

Effect of Kinase Inhibiting RNase Attenuator (KIRA) Compounds on the Formation of Face-to-Face Dimers of Inositol-Requiring Enzyme 1: Insights from Computational Modeling

Artikel/kapitelEngelska2019

Förlag, utgivningsår, omfång ...

2019-11-06
MDPI AG,2019

Nummerbeteckningar

LIBRIS-ID:oai:gup.ub.gu.se/289382
https://gup.ub.gu.se/publication/289382URI
https://doi.org/10.3390/ijms20225538DOI

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Språk:engelska

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Ämneskategori:art swepub-publicationtype

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Inositol-requiring enzyme 1 alpha (IRE1 alpha) is a transmembrane dual kinase/ribonuclease protein involved in propagation of the unfolded protein response (UPR). Inositol-requiring enzyme 1 alpha is currently being explored as a potential drug target due to the growing evidence of its role in variety of disease conditions. Upon activation, IRE1 cleaves X-box binding protein 1 (XBP1) mRNA through its RNase domain. Small molecules targeting the kinase site are known to either increase or decrease RNase activity, but the allosteric relationship between the kinase and RNase domains of IRE1 alpha is poorly understood. Subsets of IRE1 kinase inhibitors (known as "KIRA" compounds) bind to the ATP-binding site and allosterically impede the RNase activity. The KIRA compounds are able to regulate the RNase activity by stabilizing the monomeric form of IRE1 alpha. In the present work, computational analysis, protein-protein and protein-ligand docking studies, and molecular dynamics simulations were applied to different IRE1 dimer systems to provide structural insights into the perturbation of IRE1 dimers by small molecules kinase inhibitors that regulate the RNase activity. By analyzing structural deviations, energetic components, and the number of hydrogen bonds in the interface region, we propose that the KIRA inhibitors act at an early stage of IRE1 activation by interfering with IRE1 face-to-face dimer formation thus disabling the activation of the RNase domain. This work sheds light on the mechanism of action of KIRA compounds and may assist in development of further compounds in, for example, cancer therapeutics. The work also provides information on the sequence of events and protein-protein interactions initiating the unfolded protein response.

Ämnesord och genrebeteckningar

NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
IRE1
KIRA
dimer formation
unfolded protein response
protein-protein
docking
molecular dynamics simulations
allosteric inhibition
protein
ire1-alpha
efficient
dynamics
docking
server
Biochemistry & Molecular Biology
Chemistry

Biuppslag (personer, institutioner, konferenser, titlar ...)

Chintha, C. (författare)
Gorman, A. M. (författare)
Samali, A. (författare)
Eriksson, Leif A,1964Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xeleif (författare)
Göteborgs universitetInstitutionen för kemi och molekylärbiologi (creator_code:org_t)

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Ingår i:International Journal of Molecular Sciences: MDPI AG20:221422-0067

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Av författaren/redakt...: Carlesso, Antoni ...; Chintha, C.; Gorman, A. M.; Samali, A.; Eriksson, Leif A ...

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NATURVETENSKAP: NATURVETENSKAP; och Biologi; och Biokemi och mole ...

Artiklar i publikationen: International Jo ...

Av lärosätet: Göteborgs universitet

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