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Absolute yeast mito...
Absolute yeast mitochondrial proteome quantification reveals trade-off between biosynthesis and energy generation during diauxic shift
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- Di Bartolomeo, Francesca, 1986 (författare)
- Sintef Foundation for Scientific and Industrial Research At the Norwegian Institute of Technology,Chalmers tekniska högskola,Chalmers University of Technology
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- Malina, Carl, 1992 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Campbell, Kate, 1987 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Mormino, Maurizio, 1988 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine
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- Fuchs, Johannes (författare)
- Gothenburg University,Göteborgs universitet,Core Facilities, Proteomics,Core Facilities, Proteomics,University of Gothenburg
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- Vorontsov, Egor, 1988 (författare)
- Gothenburg University,Göteborgs universitet,Core Facilities, Proteomics,Core Facilities, Proteomics,University of Gothenburg
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- Gustafsson, Claes M, 1966 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine
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- Nielsen, Jens B, 1962 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology,BioInnovation Institute (BII),Danmarks Tekniske Universitet,Technical University of Denmark
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(creator_code:org_t)
- 2020-03-17
- 2020
- Engelska.
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 117:13, s. 7524-7535
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Abstract
Ämnesord
Stäng
- Saccharomyces cerevisiae constitutes a popular eukaryal model for research on mitochondrial physiology. Being Crabtree-positive, this yeast has evolved the ability to ferment glucose to ethanol and respire ethanol once glucose is consumed. Its transition phase from fermentative to respiratory metabolism, known as the diauxic shift, is reflected by dramatic rearrangements of mitochondrial function and structure. To date, the metabolic adaptations that occur during the diauxic shift have not been fully characterized at the organelle level. In this study, the absolute proteome of mitochondria was quantified alongside precise parametrization of biophysical properties associated with the mitochondrial network using state-of-the-art optical-imaging techniques. This allowed the determination of absolute protein abundances at a subcellular level. By tracking the transformation of mitochondrial mass and volume, alongside changes in the absolute mitochondrial proteome allocation, we could quantify how mitochondria balance their dual role as a biosynthetic hub as well as a center for cellular respiration. Furthermore, our findings suggest that in the transition from a fermentative to a respiratory metabolism, the diauxic shift represents the stage where major structural and functional reorganizations in mitochondrial metabolism occur. This metabolic transition, initiated at the mitochondria level, is then extended to the rest of the yeast cell.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
Nyckelord
- mitochondria
- absolute proteomics
- Saccharomyces cerevisiae
- diauxic
- shift
- inner membrane
- gene
- phosphatidylethanolamine
- cardiolipin
- biogenesis
- morphology
- proteins
- dynamics
- insights
- density
- Science & Technology - Other Topics
- Diauxic shift
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Di Bartolomeo, F ...
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Malina, Carl, 19 ...
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Campbell, Kate, ...
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Mormino, Maurizi ...
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Fuchs, Johannes
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Vorontsov, Egor, ...
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Gustafsson, Clae ...
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Nielsen, Jens B, ...
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och Biologi
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Fysiologi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Fysik
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och Annan fysik
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Proceedings of t ...
- Av lärosätet
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Göteborgs universitet
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Chalmers tekniska högskola