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Cellular organizati...
Cellular organization and histogenesis of adenosquamous carcinoma of the pancreas: evidence supporting the squamous metaplasia concept.
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Boecker, W. (author)
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Tiemann, K. (author)
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Boecker, J. (author)
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Toma, M. (author)
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Muders, M. H. (author)
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Löning, T. (author)
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Buchwalow, I. (author)
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Oldhafer, K. J. (author)
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Neumann, U. (author)
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Feyerabend, B. (author)
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- Fehr, Andre (author)
- Gothenburg University,Göteborgs universitet,Sahlgrenska Cancer Center
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- Stenman, Göran, 1953 (author)
- Gothenburg University,Göteborgs universitet,Sahlgrenska Cancer Center
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(creator_code:org_t)
- 2020-03-13
- 2020
- English.
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In: Histochemistry and Cell Biology. - : Springer Science and Business Media LLC. - 0948-6143 .- 1432-119X. ; 154:1, s. 97-105
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https://link.springe...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
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- Adenosquamous carcinoma of the pancreas (ASCAP) is characterized by conventional pancreatic ductal adenocarcinoma (PDAC) and squamous carcinoma components with at least 30% of the tumour showing squamous differentiation. To get further insight into the histogenesis of these lesions, we analysed the cellular organization of ASCAP compared to PDACs. Using Immunohistochemistry and triple immunofluorescence labelling studies for keratins, p63, p40, MUC1, MUC2, MUC5AC, Ki67, and EGFR we demonstrate that many ASCAPs contain a transitional zone between the K8/18-positive adenocarcinomatous component and the p63+/p40+/K5/K14+squamous component initiated by the expression of p63 in K8/18+adenocarcinomatous cells and the appearance of basally located p63+K5/14+cells. p63+K5/14+cells give rise to fully developed squamous differentiation. Notably, 25% of conventional PDACs without histologically recognizable squamous component contain foci of p63+p40+and K5/14+cells similar to the transitional zone. Our data provide evidence that the squamous carcinoma components of ASCAPs originate from pre-existing PDAC via transdifferentiation of keratin K8/18-positive glandular cells to p63-, p40-, and keratin K5/14-positive squamous carcinoma cells supporting the squamous metaplasia hypothesis. Thus our findings provide new evidence about the cellular process behind squamous differentiation in ASCAPs.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- Adenosquamous carcinoma
- Keratins
- Ki67
- MUC1
- MUC5AC
- Pancreas
- Transdifferentiation
- p40
- p63
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Boecker, W.
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Tiemann, K.
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Boecker, J.
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Toma, M.
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Muders, M. H.
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Löning, T.
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show more...
-
Buchwalow, I.
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Oldhafer, K. J.
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Neumann, U.
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Feyerabend, B.
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Fehr, Andre
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Stenman, Göran, ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Histochemistry a ...
- By the university
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University of Gothenburg