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Free fatty acid rec...
Free fatty acid receptor 4 activation protects against choroidal neovascularization in mice
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Tomita, Y. (författare)
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Cakir, B. (författare)
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Liu, C. H. (författare)
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visa fler...
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Fu, Z. (författare)
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Huang, S. (författare)
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Cho, S. S. (författare)
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Britton, W. R. (författare)
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Sun, Y. (författare)
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Puder, M. (författare)
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- Hellström, Ann, 1959 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology
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Talukdar, S. (författare)
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Smith, L. E. H. (författare)
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visa färre...
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(creator_code:org_t)
- 2020-03-05
- 2020
- Engelska.
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Ingår i: Angiogenesis. - : Springer Science and Business Media LLC. - 0969-6970 .- 1573-7209. ; 23, s. 385-394
- Relaterad länk:
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https://www.ncbi.nlm...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- To examine whether free fatty acid receptor 4 (FFAR4) activation can protect against choroidal neovascularization (CNV), which is a common cause of blindness, and to elucidate the mechanism underlying the inhibition, we used the mouse model of laser-induced CNV to mimic angiogenic aspects of age-related macular degeneration (AMD). Laser-induced CNV was compared between groups treated with an FFAR4 agonist or vehicle, and between FFAR4 wild-type (Ffar4+/+) and knock out (Ffar4−/−) mice on a C57BL/6J/6N background. The ex vivo choroid-sprouting assay, including primary retinal pigment epithelium (RPE) and choroid, without retina was used to investigate whether FFAR4 affects choroidal angiogenesis. Western blotting for pNF-ĸB/NF-ĸB and qRT-PCR for Il-6, Il-1β, Tnf-α, Vegf, and Nf-ĸb were used to examine the influence of FFAR4 on inflammation, known to influence CNV. RPE isolated from Ffar4+/+ and Ffar4−/− mice were used to assess RPE contribution to inflammation. The FFAR4 agonist suppressed laser-induced CNV in C57BL/6J mice, and CNV increased in Ffar4−/− compared to Ffar4+/+ mice. We showed that the FFAR4 agonist acted through the FFAR4 receptor. The FFAR4 agonist suppressed mRNA expression of inflammation markers (Il-6, Il-1β) via the NF-ĸB pathway in the retina, choroid, RPE complex. The FFAR4 agonist suppressed neovascularization in the choroid-sprouting ex vivo assay and FFAR4 deficiency exacerbated sprouting. Inflammation markers were increased in primary RPE cells of Ffar4−/− mice compared with Ffar4+/+ RPE. In this mouse model, the FFAR4 agonist suppressed CNV, suggesting FFAR4 to be a new molecular target to reduce pathological angiogenesis in CNV. © 2020, Springer Nature B.V.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Nyckelord
- Age-related macular degeneration (AMD)
- Free fatty acid receptor 4 (FFAR4)
- IL-6
- Laser-induced choroidal neovascularization (CNV)
- NF-ĸb
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Tomita, Y.
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Cakir, B.
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Liu, C. H.
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Fu, Z.
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Huang, S.
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Cho, S. S.
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visa fler...
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Britton, W. R.
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Sun, Y.
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Puder, M.
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Hellström, Ann, ...
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Talukdar, S.
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Smith, L. E. H.
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visa färre...
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- MEDICIN OCH HÄLSOVETENSKAP
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- Artiklar i publikationen
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Angiogenesis
- Av lärosätet
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Göteborgs universitet