Fast-tracking of single emitters in large volumes with nanometer precision

• Multifocal plane microscopy allows for capturing images at different focal planes simultaneously. Using a proprietary prism which splits the emitted light into paths of different lengths, images at 8 different focal depths were obtained, covering a volume of 50x50x4 mu m(3). The position of single emitters was retrieved using a phasor-based approach across the different imaging planes, with better than 10 nm precision in the axial direction. We validated the accuracy of this approach by tracking fluorescent beads in 3D to calculate water viscosity. The fast acquisition rate (>100 fps) also enabled us to follow the capturing of 0.2 mu m fluorescent beads into an optical trap. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)

Nyckelord

3-dimensional particle tracking

image correlation spectroscopy

multifocal plane microscopy

fluorescence microscopy

localization

microscopy

molecule localization

cell

dynamics

diffusion

receptor

Optics

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)