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First amyloid β1-42...
First amyloid β1-42 certified reference material for re-calibrating commercial immunoassays
- Article/chapterEnglish2020
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Numbers
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LIBRIS-ID:oai:gup.ub.gu.se/299390
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https://gup.ub.gu.se/publication/299390URI
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https://doi.org/10.1002/alz.12145DOI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Introduction: Reference materials based on human cerebrospinal fluid were certified for the mass concentration of amyloid beta (Aβ)1-42 (Aβ42). They are intended to be used to calibrate diagnostic assays for Aβ42. Methods: The three certified reference materials (CRMs), ERM-DA480/IFCC, ERM-DA481/IFCC and ERM-DA482/IFCC, were prepared at three concentration levels and characterized using isotope dilution mass spectrometry methods. Roche, EUROIMMUN, and Fujirebio used the three CRMs to re-calibrate their immunoassays. Results: The certified Aβ42 mass concentrations in ERM-DA480/IFCC, ERM-DA481/IFCC, and ERM-DA482/IFCC are 0.45, 0.72, and 1.22μg/L, respectively, with expanded uncertainties (k=2) of 0.07, 0.11, and 0.18μg/L, respectively. Before re-calibration, a good correlation (Pearson's r>0.97), yet large biases, were observed between results from different commercial assays. After re-calibration the between-assay bias was reduced to<5%. Discussion: The Aβ42 CRMs can ensure the equivalence of results between methods and across platforms for the measurement of Aβ42. © 2020 the Alzheimer's Association
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Kuhlmann, J.
(author)
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Andreasson, Ulf,1968Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry(Swepub:gu)xaulfz
(author)
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Brix, B.
(author)
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Venkataraman, I.
(author)
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Herbst, V.
(author)
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Rutz, S.
(author)
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Manuilova, E.
(author)
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Vandijck, M.
(author)
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Dekeyser, F.
(author)
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Bjerke, Maria,1977Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry(Swepub:gu)xsmare
(author)
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Pannee, Josef,1979Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry(Swepub:gu)xpajos
(author)
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Charoud-Got, J.
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Auclair, G.
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Mazoua, S.
(author)
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Pinski, G.
(author)
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Trapmann, S.
(author)
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Schimmel, H.
(author)
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Emons, H.
(author)
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Quaglia, M.
(author)
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Portelius, Erik,1977Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry(Swepub:gu)xporer
(author)
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Korecka, M.
(author)
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Shaw, L. M.
(author)
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Lame, M.
(author)
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Chambers, E.
(author)
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Vanderstichele, H.
(author)
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Stoops, E.
(author)
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Leinenbach, A.
(author)
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Bittner, T.
(author)
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Jenkins, R. G.
(author)
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Kostanjevecki, V.
(author)
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Lewczuk, P.
(author)
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Gobom, JohanGothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry(Swepub:gu)xgobjo
(author)
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Zetterberg, Henrik,1973Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry(Swepub:gu)xzethe
(author)
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Zegers, I.
(author)
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Blennow, Kaj,1958Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry(Swepub:gu)xbleka
(author)
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Göteborgs universitetInstitutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi
(creator_code:org_t)
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In:Alzheimer's and Dementia: Wiley16:11, s. 1493-15031552-52601552-5279
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Boulo, S.
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Kuhlmann, J.
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Andreasson, Ulf, ...
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Brix, B.
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Venkataraman, I.
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Herbst, V.
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Rutz, S.
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Manuilova, E.
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Vandijck, M.
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Dekeyser, F.
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Bjerke, Maria, 1 ...
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Pannee, Josef, 1 ...
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Charoud-Got, J.
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Auclair, G.
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Mazoua, S.
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Pinski, G.
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Trapmann, S.
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Schimmel, H.
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Emons, H.
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Quaglia, M.
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Portelius, Erik, ...
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Korecka, M.
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Shaw, L. M.
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Lame, M.
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Chambers, E.
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Vanderstichele, ...
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Stoops, E.
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Leinenbach, A.
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Bittner, T.
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Jenkins, R. G.
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Kostanjevecki, V ...
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Lewczuk, P.
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Gobom, Johan
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Zetterberg, Henr ...
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Zegers, I.
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Blennow, Kaj, 19 ...
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