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  • Boulo, S. (author)

First amyloid β1-42 certified reference material for re-calibrating commercial immunoassays

  • Article/chapterEnglish2020

Publisher, publication year, extent ...

  • 2020-08-04
  • Wiley,2020

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/299390
  • https://gup.ub.gu.se/publication/299390URI
  • https://doi.org/10.1002/alz.12145DOI

Supplementary language notes

  • Language:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Introduction: Reference materials based on human cerebrospinal fluid were certified for the mass concentration of amyloid beta (Aβ)1-42 (Aβ42). They are intended to be used to calibrate diagnostic assays for Aβ42. Methods: The three certified reference materials (CRMs), ERM-DA480/IFCC, ERM-DA481/IFCC and ERM-DA482/IFCC, were prepared at three concentration levels and characterized using isotope dilution mass spectrometry methods. Roche, EUROIMMUN, and Fujirebio used the three CRMs to re-calibrate their immunoassays. Results: The certified Aβ42 mass concentrations in ERM-DA480/IFCC, ERM-DA481/IFCC, and ERM-DA482/IFCC are 0.45, 0.72, and 1.22μg/L, respectively, with expanded uncertainties (k=2) of 0.07, 0.11, and 0.18μg/L, respectively. Before re-calibration, a good correlation (Pearson's r>0.97), yet large biases, were observed between results from different commercial assays. After re-calibration the between-assay bias was reduced to<5%. Discussion: The Aβ42 CRMs can ensure the equivalence of results between methods and across platforms for the measurement of Aβ42. © 2020 the Alzheimer's Association

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Kuhlmann, J. (author)
  • Andreasson, Ulf,1968Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry(Swepub:gu)xaulfz (author)
  • Brix, B. (author)
  • Venkataraman, I. (author)
  • Herbst, V. (author)
  • Rutz, S. (author)
  • Manuilova, E. (author)
  • Vandijck, M. (author)
  • Dekeyser, F. (author)
  • Bjerke, Maria,1977Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry(Swepub:gu)xsmare (author)
  • Pannee, Josef,1979Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry(Swepub:gu)xpajos (author)
  • Charoud-Got, J. (author)
  • Auclair, G. (author)
  • Mazoua, S. (author)
  • Pinski, G. (author)
  • Trapmann, S. (author)
  • Schimmel, H. (author)
  • Emons, H. (author)
  • Quaglia, M. (author)
  • Portelius, Erik,1977Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry(Swepub:gu)xporer (author)
  • Korecka, M. (author)
  • Shaw, L. M. (author)
  • Lame, M. (author)
  • Chambers, E. (author)
  • Vanderstichele, H. (author)
  • Stoops, E. (author)
  • Leinenbach, A. (author)
  • Bittner, T. (author)
  • Jenkins, R. G. (author)
  • Kostanjevecki, V. (author)
  • Lewczuk, P. (author)
  • Gobom, JohanGothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry(Swepub:gu)xgobjo (author)
  • Zetterberg, Henrik,1973Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry(Swepub:gu)xzethe (author)
  • Zegers, I. (author)
  • Blennow, Kaj,1958Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry(Swepub:gu)xbleka (author)
  • Göteborgs universitetInstitutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi (creator_code:org_t)

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