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Longitudinal Plasma...
Longitudinal Plasma Protein Profiling Using Targeted Proteomics and Recombinant Protein Standards
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- Kotol, David (författare)
- KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab
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- Hunt, Helian (författare)
- KTH,Skolan för kemi, bioteknologi och hälsa (CBH),Science for Life Laboratory, SciLifeLab
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- Hober, Andreas (författare)
- KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab
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- Karlsson, Max J. (författare)
- KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab
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- Forsström, Björn (författare)
- KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab
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- Gummesson, Anders, 1973 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine,Univ Gothenburg, Sahlgrenska Acad, Dept Mol & Clin Med, SE-40530 Gothenburg, Sweden.
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- Bergström, Göran, 1964 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine,Univ Gothenburg, Sahlgrenska Acad, Dept Mol & Clin Med, SE-40530 Gothenburg, Sweden.
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- Fagerberg, L. (författare)
- Sci Life Lab, SE-17165 Solna, Sweden.
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- Uhlén, Mathias (författare)
- KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab,Tech Univ Denmark, Novo Nordisk Fdn Ctr Biosustainabil, DK-2970 Horsholm, Denmark.
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- Edfors, Fredrik (författare)
- KTH,Proteinvetenskap,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- 2020-08-21
- 2020
- Engelska.
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Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 19:12, s. 4815-4825
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Spike-in of standards of known concentrations used in proteomics-based workflows is an attractive approach for both accurate and precise multiplexed protein quantification. Here, a quantitative method based on targeted proteomics analysis of plasma proteins using isotope-labeled recombinant standards originating from the Human Protein Atlas project has been established. The standards were individually quantified prior to being employed in the final multiplex assay. The assays are mainly directed toward actively secreted proteins produced in the liver, but may also originate from other parts of the human body. This study included 21 proteins classified by the FDA as either drug targets or approved clinical protein biomarkers. We describe the use of this multiplex assay for profiling a well-defined human cohort with sample collection spanning over a one-year period. Samples were collected at four different time points, which allowed for a longitudinal analysis to assess the variable plasma proteome within individuals. Two assays toward APOA1 and APOB had available clinical data, and the two assays were benchmarked against each other. The clinical assay is based on antibodies and shows high correlation between the two orthogonal methods, suggesting that targeted proteomics with highly parallel, multiplex analysis is an attractive alternative to antibody-based protein assays.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- mass spectrometry
- proteomics
- blood plasma
- stable isotope standards
- absolute quantification
- recombinant protein standards
- precision
- medicine
- expression
- peptide
- assays
- aim
- Biochemistry & Molecular Biology
- mass spectrometry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Kotol, David
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Hunt, Helian
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Hober, Andreas
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Karlsson, Max J.
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Forsström, Björn
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Gummesson, Ander ...
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visa fler...
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Bergström, Göran ...
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Fagerberg, L.
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Uhlén, Mathias
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Edfors, Fredrik
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
- Artiklar i publikationen
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Journal of Prote ...
- Av lärosätet
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Göteborgs universitet
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Kungliga Tekniska Högskolan