Search: WFRF:(Barchiesi Riccardo) >
Downregulation of S...
Downregulation of Synaptotagmin 1 in the Prelimbic Cortex Drives Alcohol-Associated Behaviors in Rats
-
- Barbier, Estelle (author)
- Linköpings universitet,Centrum för social och affektiv neurovetenskap,Medicinska fakulteten
-
- Barchiesi, Riccardo (author)
- Linköpings universitet,Centrum för social och affektiv neurovetenskap,Medicinska fakulteten
-
- Domi, Ana, 1990 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry,Univ Gothenburg, Sweden
-
show more...
-
- Chanthongdee, Kanat (author)
- Linköpings universitet,Centrum för social och affektiv neurovetenskap,Medicinska fakulteten,Mahidol Univ, Thailand
-
- Domi, Esi (author)
- Linköpings universitet,Centrum för social och affektiv neurovetenskap,Medicinska fakulteten
-
- Augier, Gaëlle (author)
- Linköpings universitet,Centrum för social och affektiv neurovetenskap,Medicinska fakulteten
-
- Augier, Eric (author)
- Linköpings universitet,Centrum för social och affektiv neurovetenskap,Medicinska fakulteten
-
- Xu, Li (author)
- Linköpings universitet,Centrum för social och affektiv neurovetenskap,Medicinska fakulteten,Sichuan Prov Peoples Hosp, Peoples R China
-
- Adermark, Louise, 1974 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry,Univ Gothenburg, Sweden
-
- Heilig, Markus (author)
- Linköpings universitet,Centrum för social och affektiv neurovetenskap,Medicinska fakulteten,Region Östergötland, Psykiatriska kliniken i Linköping
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2021
- 2021
- English.
-
In: Biological Psychiatry. - : Elsevier BV. - 0006-3223 .- 1873-2402. ; 89:4, s. 398-406
- Related links:
-
http://www.biologica...
-
show more...
-
https://liu.diva-por... (primary) (Raw object)
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Background: Alcohol addiction is characterized by persistent neuroadaptations in brain structures involved in motivation, emotion, and decision making, including the medial prefrontal cortex, the nucleus accumbens, and the amygdala. We previously reported that induction of alcohol dependence was associated with long-term changes in the expression of genes involved in neurotransmitter release. Specifically, Syt1, which plays a key role in neurotransmitter release and neuronal functions, was downregulated. Here, we therefore examined the role of Syt1 in alcohol-associated behaviors in rats. Methods: We evaluated the effect of Syt1 downregulation using an adeno-associated virus (AAV) containing a short hairpin RNA against Syt1. Cre-dependent Syt1 was also used in combination with an rAAV2 retro-Cre virus to assess circuit-specific effects of Syt1 knockdown (KD). Results: Alcohol-induced downregulation of Syt1 is specific to the prelimbic cortex (PL), and KD of Syt1 in the PL resulted in escalated alcohol consumption, increased motivation to consume alcohol, and increased alcohol drinking despite negative consequences (“compulsivity”). Syt1 KD in the PL altered the excitation/inhibition balance in the basolateral amygdala, while the nucleus accumbens core was unaffected. Accordingly, a projection-specific Syt1 KD in the PL–basolateral amygdala projection was sufficient to increase compulsive alcohol drinking, while a KD of Syt1 restricted to PL–nucleus accumbens core projecting neurons had no effect on tested alcohol-related behaviors. Conclusions: Together, these data suggest that dysregulation of Syt1 is an important mechanism in long-term neuroadaptations observed after a history of alcohol dependence, and that Syt1 regulates alcohol-related behaviors in part by affecting a PL–basolateral amygdala brain circuit. © 2020 Society of Biological Psychiatry
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Keyword
- Alcohol addiction
- Basolateral amygdala
- Compulsivity
- Nucleus accumbens
- Prelimbic cortex
- Syt1
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Barbier, Estelle
-
Barchiesi, Ricca ...
-
Domi, Ana, 1990
-
Chanthongdee, Ka ...
-
Domi, Esi
-
Augier, Gaëlle
-
show more...
-
Augier, Eric
-
Xu, Li
-
Adermark, Louise ...
-
Heilig, Markus
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Neurosciences
- Articles in the publication
-
Biological Psych ...
- By the university
-
University of Gothenburg
-
Linköping University