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Molecular Character...
Molecular Characterization of the Enterohemolysin Gene (ehxA) in Clinical Shiga Toxin-Producing Escherichia coli Isolates
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- Hua, Y. (author)
- Southern Med Univ, Peoples R China; Karolinska Inst, Sweden
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- Zhang, J. (author)
- Massey Univ, New Zealand
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- Jernberg, C. (author)
- Publ Hlth Agcy Sweden, Sweden
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- Chromek, M. (author)
- Karolinska Institutet,Karolinska Inst, Sweden; Karolinska Univ Hosp, Sweden
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- Hansson, Sverker, 1946 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics,Sahlgrens Univ Hosp, Sweden; Univ Gothenburg, Sweden
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- Frykman, Anne, 1983 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för pediatrik,Institute of Clinical Sciences, Department of Pediatrics,Sahlgrens Univ Hosp, Sweden; Univ Gothenburg, Sweden
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- Xiong, Y. W. (author)
- Chinese Ctr Dis Control & Prevent, Peoples R China
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- Wan, C. S. (author)
- Southern Med Univ, Peoples R China
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- Matussek, Andreas (author)
- Linköpings universitet,Karolinska Institutet,Avdelningen för inflammation och infektion,Medicinska fakulteten,Karolinska Inst, Sweden; Oslo Univ Hosp, Norway; Univ Oslo, Norway
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- Bai, X. N. (author)
- Karolinska Institutet,Chinese Ctr Dis Control & Prevent, Peoples R China; Karolinska Inst, Sweden
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(creator_code:org_t)
- 2021-01-19
- 2021
- English.
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In: Toxins. - : MDPI AG. - 2072-6651. ; 13:1
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Abstract
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- Shiga toxin (Stx)-producing Escherichia coli (STEC) is an important foodborne pathogen with the ability to cause bloody diarrhea (BD) and hemolytic uremic syndrome (HUS). Little is known about enterohemolysin-encoded by ehxA. Here we investigated the prevalence and diversity of ehxA in 239 STEC isolates from human clinical samples. In total, 199 out of 239 isolates (83.26%) were ehxA positive, and ehxA was significantly overrepresented in isolates carrying stx(2a) + stx(2c) (p < 0.001) and eae (p < 0.001). The presence of ehxA was significantly associated with BD and serotype O157:H7. Five ehxA subtypes were identified, among which, ehxA subtypes B, C, and F were overrepresented in eae-positive isolates. All O157:H7 isolates carried ehxA subtype B, which was related to BD and HUS. Three ehxA groups were observed in the phylogenetic analysis, namely, group ? (ehxA subtype A), group II (ehxA subtype B, C, and F), and group (ehxA subtype D). Most BD- and HUS-associated isolates were clustered into ehxA group II, while ehxA group ? was associated with non-bloody stool and individuals >= 10 years of age. The presence of ehxA + eae and ehxA + eae + stx(2) was significantly associated with HUS and O157:H7 isolates. In summary, this study showed a high prevalence and the considerable genetic diversity of ehxA among clinical STEC isolates. The ehxA genotypes (subtype B and phylogenetic group II) could be used as risk predictors, as they were associated with severe clinical symptoms, such as BD and HUS. Furthermore, ehxA, together with stx and eae, can be used as a risk predictor for HUS in STEC infections.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Keyword
- Shiga toxin-producing Escherichia coli
- enterohemolysin
- ehxA
- gene
- diversity
- hemolytic uremic syndrome
- clinical significance
- Food Science & Technology
- Toxicology
- Shiga toxin-producing Escherichia coli; enterohemolysin; ehxA; gene diversity; hemolytic uremic syndrome; clinical significance
Publication and Content Type
- ref (subject category)
- art (subject category)
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Toxins
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- By the author/editor
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Hua, Y.
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Zhang, J.
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Jernberg, C.
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Chromek, M.
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Hansson, Sverker ...
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Frykman, Anne, 1 ...
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show more...
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Xiong, Y. W.
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Wan, C. S.
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Matussek, Andrea ...
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Bai, X. N.
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Microbiology
- Articles in the publication
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Toxins
- By the university
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University of Gothenburg
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Karolinska Institutet
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Linköping University