Long-read-sequenced reference genomes of the seven major lineages of enterotoxigenic Escherichia coli (ETEC) circulating in modern time

von Mentzer, Astrid,1983Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology,University of Gothenburg,Wellcome Trust Sanger Institute,Chalmers tekniska högskola,Chalmers University of Technology (author)

Enterotoxigenic Escherichia coli (ETEC) is an enteric pathogen responsible for the majority of diarrheal cases worldwide. ETEC infections are estimated to cause 80,000 deaths annually, with the highest rates of burden, ca 75 million cases per year, amongst children under 5 years of age in resource-poor countries. It is also the leading cause of diarrhoea in travellers. Previous large-scale sequencing studies have found seven major ETEC lineages currently in circulation worldwide. We used PacBio long-read sequencing combined with Illumina sequencing to create high-quality complete reference genomes for each of the major lineages with manually curated chromosomes and plasmids. We confirm that the major ETEC lineages all harbour conserved plasmids that have been associated with their respective background genomes for decades, suggesting that the plasmids and chromosomes of ETEC are both crucial for ETEC virulence and success as pathogens. The in-depth analysis of gene content, synteny and correct annotations of plasmids will elucidate other plasmids with and without virulence factors in related bacterial species. These reference genomes allow for fast and accurate comparison between different ETEC strains, and these data will form the foundation of ETEC genomics research for years to come.

Blackwell, G. A.European Bioinformatics Institute,Wellcome Trust Sanger Institute (author)
Pickard, D.University Of Cambridge (author)
Boinett, C. J.Wellcome Trust Sanger Institute (author)
Joffre, E.Karolinska Institutet (author)
Page, A. J.Wellcome Trust Sanger Institute (author)
Svennerholm, Ann-Mari,1947Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology,University of Gothenburg(Swepub:gu)xsannx (author)
Dougan, G.University Of Cambridge (author)
Sjoling, A.Karolinska Institutet (author)
Göteborgs universitetInstitutionen för biomedicin, avdelningen för mikrobiologi och immunologi (creator_code:org_t)

By the author/editor: von Mentzer, Ast ...; Blackwell, G. A.; Pickard, D.; Boinett, C. J.; Joffre, E.; Page, A. J.; show more...; Svennerholm, Ann ...; Dougan, G.; Sjoling, A.; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Immunology in th ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Infectious Medic ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Microbiology in ...

By the university: University of Gothenburg; Chalmers University of Technology; Karolinska Institutet

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