Sökning: WFRF:(Jansson Jessica) >
Bacterial Compositi...
Bacterial Composition and Metabolomics of Dental Plaque From Adolescents
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- Havsed, Kristian (författare)
- Jönköping University,Malmö universitet,Odontologiska fakulteten (OD),Department of Pediatric Dentistry, Institute for Postgraduate Dental Education, Jönköping, Sweden; Centre for Oral Health, School of Health and Welfare, Jönköping University, Jönköping, Sweden,HHJ. Centrum för oral hälsa,Inst Postgrad Dent Educ, Dept Pediat Dent, Jonkoping, Sweden.; Fac Odontol, Sect Oral Biol & Pathol, Malmo, Sweden.
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- Stensson, Malin, 1970- (författare)
- Jönköping University,HHJ, Avdelningen för naturvetenskap och biomedicin,HHJ. Centrum för oral hälsa,HHJ. CHILD
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- Jansson, Henrik, 1971- (författare)
- Jönköping University,Malmö universitet,Odontologiska fakulteten (OD),Centre for Oral Health, School of Health and Welfare, Jönköping University, Jönköping, Sweden; Folktandvården Skåne, The Swedish Dental Service of Skåne, Lund, Sweden,HHJ, Avdelningen för naturvetenskap och biomedicin,HHJ. Centrum för oral hälsa,HHJ. ARN-J (Aging Research Network - Jönköping)
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- Carda-Diéguez, Miguel (författare)
- Department of Health & Genomics, Foundation for the Promotion of Health and Biomedical Research (FISABIO) Foundation, Valencia, Spain,Fdn Promot Hlth & Biomed Res FISABIO Fdn, Dept Hlth & Genom, Valencia, Spain.
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- Pedersen, Anders, 1976 (författare)
- Gothenburg University,Göteborgs universitet,Svenskt NMR-centrum vid Göteborgs universitet,Swedish NMR Centre at Göteborg University,Swedish NMR Centre, The University of Gothenburg, Gothenburg, Sweden,Univ Gothenburg, Swedish NMR Ctr, Gothenburg, Sweden.
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- Neilands, Jessica (författare)
- Malmö universitet,Odontologiska fakulteten (OD),Biofilms Research Center for Biointerfaces,Malmo Univ, Fac Odontol, Sect Oral Biol & Pathol, Malmo, Sweden.;Malmo Univ, Biofilms Res Ctr Biointerfaces, Malmo, Sweden.
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- Svensäter, Gunnel, 1954- (författare)
- Malmö universitet,Odontologiska fakulteten (OD),Biofilms Research Center for Biointerfaces,Malmo Univ, Fac Odontol, Sect Oral Biol & Pathol, Malmo, Sweden.;Malmo Univ, Biofilms Res Ctr Biointerfaces, Malmo, Sweden.
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- Mira, Alex (författare)
- Jönköping University,HHJ. Centrum för oral hälsa,HHJ, Avdelningen för naturvetenskap och biomedicin,Department of Health & Genomics, Foundation for the Promotion of Health and Biomedical Research (FISABIO) Foundation, Valencia, Spain
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(creator_code:org_t)
- 2021-07-30
- 2021
- Engelska.
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Ingår i: Frontiers in Cellular and Infection Microbiology. - : Frontiers Media SA. - 2235-2988. ; 11
- Relaterad länk:
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https://www.frontier...
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https://doi.org/10.3...
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https://mau.diva-por... (primary) (Raw object)
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https://gup.ub.gu.se...
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https://doi.org/10.3...
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https://urn.kb.se/re...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Supragingival dental plaque samples were collected from 40 Swedish adolescents, including 20 with caries lesions (CAR) and 20 caries-free (CF). Fresh plaque samples were subjected to an ex vivo acid tolerance (AT) test where the proportion of bacteria resistant to an acid shock was evaluated through confocal microscopy and live/dead staining, and the metabolites produced were quantified by 1H Nuclear Magnetic Resonance (1H NMR). In addition, DNA was extracted and the 16S rRNA gene was sequenced by Illumina sequencing, in order to characterize bacterial composition in the same samples. There were no significant differences in AT scores between CAR and CF individuals. However, 7 out of the 10 individuals with highest AT scores belonged to the CAR group. Regarding bacterial composition, Abiotrophia, Prevotella and Veillonella were found at significantly higher levels in CAR individuals (p=0.0085, 0.026 and 0.04 respectively) and Rothia and Corynebacterium at significantly higher levels in CF individuals (p=0.026 and 0.003). The caries pathogen Streptococcus mutans was found at low frequencies and was absent in 60% of CAR individuals. Random-forest predictive models indicate that at least 4 bacterial species or 9 genera are needed to distinguish CAR from CF adolescents. The metabolomic profile obtained by NMR showed a significant clustering of organic acids with specific bacteria in CAR and/or high AT individuals, being Scardovia wiggsiae the species with strongest associations. A significant clustering of ethanol and isopropanol with health-associated bacteria such as Rothia or Corynebacterium was also found. Accordingly, several relationships involving these compounds like the Ethanol : Lactate or Succinate : Lactate ratios were significantly associated to acid tolerance and could be of predictive value for caries risk. We therefore propose that future caries risk studies would benefit from considering not only the use of multiple organisms as potential microbial biomarkers, but also their functional adaptation and metabolic output.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Annan medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Other Medical Biotechnology (hsv//eng)
Nyckelord
- acid tolerance
- caries risk (assessment)
- dental caries
- metabolomics (OMICS)
- microbiome
- NMR
- Rothia
- Streptococcus mutans (S. mutans)
- NMR
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Havsed, Kristian
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Stensson, Malin, ...
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Jansson, Henrik, ...
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Carda-Diéguez, M ...
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Pedersen, Anders ...
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Neilands, Jessic ...
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Svensäter, Gunne ...
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Mira, Alex
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- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Odontologi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Analytisk kemi
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Annan medicinsk ...
- Artiklar i publikationen
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Frontiers in Cel ...
- Av lärosätet
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Göteborgs universitet
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Malmö universitet
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Jönköping University