Concepts to Reveal Parvovirus-Nucleus Interactions

• Parvoviruses are small single-stranded (ss) DNA viruses, which replicate in the nucleoplasm and affect both the structure and function of the nucleus. The nuclear stage of the parvovirus life cycle starts at the nuclear entry of incoming capsids and culminates in the successful passage of progeny capsids out of the nucleus. In this review, we will present past, current, and future microscopy and biochemical techniques and demonstrate their potential in revealing the dynamics and molecular interactions in the intranuclear processes of parvovirus infection. In particular, a number of advanced techniques will be presented for the detection of infection-induced changes, such as DNA modification and damage, as well as protein-chromatin interactions.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Infektionsmedicin (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Infectious Medicine (hsv//eng)

Nyckelord

parvoviruses

nucleus

imaging of viral interactions and dynamics

analysis of protein-protein interactions

analysis of virus-chromatin

interactions

nonstructural protein ns2

DNA-damage response

cell-cycle arrest

minute-virus

adenoassociated virus

canine parvovirus

capsid proteins

cytoplasmic trafficking

vp1n-terminal sequence

b19 infection

Virology

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)