Electrochemical Measurements Reveal Reactive Oxygen Species in Stress Granules**

• Stress granules (SGs) are membrane-less organelles that assemble in the cytoplasm to organize cellular contents and promote rapid adaptation during stress. To understand how SGs contribute to physiological functions, we used electrochemical measurements to detect electroactive species in SGs. With amperometry, we discovered that reactive oxygen species (ROS) are encapsulated inside arsenite-induced SGs, and H2O2 is the main species. The release kinetics of H2O2 from single SGs and the number of H2O2 molecules were quantified. The discovery that SGs contain ROS implicates them as communicators of the cellular stresses rather than a simple endpoint. This may explain how SGs regulate cellular metabolism and stress responses. This may also help better understand their cytoprotective functions in pathological conditions associated with SGs such as neurodegenerative diseases (NDs), cancers and viral infections. © 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.

Ämnesord

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

Nyckelord

amperometry

microelectrodes

neurodegeneration

reactive oxygen species

stress granules

Cytology

Hydrogen peroxide

Neurodegenerative diseases

Oxygen

Cellular metabolism

Electroactive species

Electrochemical measurements

Pathological conditions

Physiological functions

Rapid adaptation

Release kinetics

Viral infections

Granulation

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)