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Ulva fenestrata protein – comparison of three extraction methods with respect to protein yield and protein quality

Juul, Louise (author)
Århus Universitet,Aarhus University
Danielsen, Marianne (author)
Århus Universitet,Aarhus University
Nebel, Caroline (author)
Århus Universitet,Aarhus University
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Steinhagen, Sophie (author)
Gothenburg University,Göteborgs universitet,Institutionen för marina vetenskaper,Institutionen för marina vetenskaper, Tjärnö marinlaboratoriet,SWEMARC,Department of marine sciences,Department of marine sciences, Tjärnö Marine Laboratory,University of Gothenburg,Århus Universitet,Aarhus University
Bruhn, Annette (author)
Århus Universitet,Aarhus University
Krogh Jensen, Søren (author)
Undeland, Ingrid, 1968 (author)
Chalmers tekniska högskola,Chalmers University of Technology
Kastrup Dalsgaard, Trine (author)
Århus Universitet,Aarhus University
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 (creator_code:org_t)
Elsevier BV, 2021
2021
English.
In: Algal Research. - : Elsevier BV. - 2211-9264. ; 60
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • Seaweed is gaining attention as a possible alternative and sustainable source of proteins. This study investigates three protein extraction methods and their effect on protein yield and quality when applied to Ulva fenestrata . Two of the methods included alkaline extractions (pH-shifts); one version solubilizing the proteins at pH 8.5 and one solubilizing them at pH 8.5 followed by pH 12 (pH 8.5+12). The third method was a mechanical pressing, using a double screw press. All extraction methods were followed by isoelectric precipitation to concentrate the proteins. Extraction at pH 8.5 gave the significantly highest total protein yield after the isoelectric precipitation, followed by extraction at pH 8.5+12 and lastly mechanical extraction gave the lowest yield. Proteins extracted with both alkaline methods had a significantly higher solubility at pH 7 and pH 9, compared to proteins from the mechanical pressing. There were no significant differences between the three methods in total D/L-amino acid ratio. Amino acid cross-links measured as lysinoalanine (LAL) and lanthionine (LAN) where found in significantly higher amounts in alkali-extracted proteins compared to mechanically extracted, however not to a degree that expect to compromise functional or nutritional quality. Further, no significant difference in protein in vitro digestibility was found between extraction methods. In conclusion, results indicated that protein extraction at pH 8.5 can be recommended, especially regarding total protein yield and solubility of the final protein extract.

Subject headings

NATURVETENSKAP  -- Kemi (hsv//swe)
NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)
NATURVETENSKAP  -- Biologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences (hsv//eng)
TEKNIK OCH TEKNOLOGIER  -- Naturresursteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY  -- Environmental Engineering (hsv//eng)
TEKNIK OCH TEKNOLOGIER  -- Miljöbioteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY  -- Environmental Biotechnology (hsv//eng)
TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology (hsv//eng)
NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Andra medicinska och farmaceutiska grundvetenskaper (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Other Basic Medicine (hsv//eng)
TEKNIK OCH TEKNOLOGIER  -- Industriell bioteknik -- Annan industriell bioteknik (hsv//swe)
ENGINEERING AND TECHNOLOGY  -- Industrial Biotechnology -- Other Industrial Biotechnology (hsv//eng)

Keyword

Seaweed
protein extraction
pH-shift
mechanical pressing
digestibility
process-induced modifications
Seaweed

Publication and Content Type

ref (subject category)
art (subject category)

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