SwePub
Sök i LIBRIS databas

  Extended search

WFRF:(Svensson Anna C.)
 

Search: WFRF:(Svensson Anna C.) > Systematic evaluati...

  • Hober, Sophia,Professor,1965-KTH Royal Institute of Technology (author)

Systematic evaluation of SARS-CoV-2 antigens enables a highly specific and sensitive multiplex serological COVID-19 assay

  • Article/chapterEnglish2021

Publisher, publication year, extent ...

  • 2021-07-19
  • Wiley,2021

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/307424
  • https://gup.ub.gu.se/publication/307424URI
  • https://doi.org/10.1002/cti2.1312DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-299708URI
  • https://lup.lub.lu.se/record/73f69d92-0a20-4fb9-b33d-2c17fa5f0685URI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:147281877URI

Supplementary language notes

  • Language:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • QC 20210816
  • Objective. The COVID-19 pandemic poses an immense need for accurate, sensitive and high-throughput clinical tests, and serological assays are needed for both overarching epidemiological studies and evaluating vaccines. Here, we present the development and validation of a high-throughput multiplex bead-based serological assay. Methods. More than 100 representations of SARS-CoV-2 proteins were included for initial evaluation, including antigens produced in bacterial and mammalian hosts as well as synthetic peptides. The five best-performing antigens, three representing the spike glycoprotein and two representing the nucleocapsid protein, were further evaluated for detection of IgG antibodies in samples from 331 COVID-19 patients and convalescents, and in 2090 negative controls sampled before 2020. Results. Three antigens were finally selected, represented by a soluble trimeric form and the S1-domain of the spike glycoprotein as well as by the C-terminal domain of the nucleocapsid. The sensitivity for these three antigens individually was found to be 99.7%, 99.1% and 99.7%, and the specificity was found to be 98.1%, 98.7% and 95.7%. The best assay performance was although achieved when utilising two antigens in combination, enabling a sensitivity of up to 99.7% combined with a specificity of 100%. Requiring any two of the three antigens resulted in a sensitivity of 99.7% and a specificity of 99.4%. Conclusion. These observations demonstrate that a serological test based on a combination of several SARS-CoV-2 antigens enables a highly specific and sensitive multiplex serological COVID-19 assay.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Hellström, CeciliaKTH Royal Institute of Technology,KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1doy1fn (author)
  • Olofsson, JennieKTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik(Swepub:kth)u1q8hz5k (author)
  • Andersson, EniKTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik(Swepub:kth)u11gerbc (author)
  • Bergström, SofiaKTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik(Swepub:kth)u1ugb8pi (author)
  • Jernbom Falk, AugustKTH Royal Institute of Technology,KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1db8k5t (author)
  • Bayati, ShaghayeghKTH Royal Institute of Technology,KTH,Div Affin Prote, Dept Prot Sci, SciLifeLab, Stockholm, Sweden.(Swepub:kth)u1n22avk (author)
  • Mravinacová, SáraKTH Royal Institute of Technology,KTH,Proteinvetenskap(Swepub:kth)u1utu00u (author)
  • Sjöberg, RonaldKTH Royal Institute of Technology,KTH,Proteinvetenskap(Swepub:kth)u1j1wpi6 (author)
  • Yousef, JamilKTH Royal Institute of Technology,KTH,Proteinvetenskap(Swepub:kth)u19brnwx (author)
  • Skoglund, LovisaKarolinska Institutet,KTH Royal Institute of Technology,KTH,Proteinvetenskap(Swepub:kth)u1icrrm2 (author)
  • Kanje, SaraKTH Royal Institute of Technology,KTH,Proteinvetenskap(Swepub:kth)u1t204cn (author)
  • Berling, AnnaKTH Royal Institute of Technology,KTH,Proteinvetenskap(Swepub:kth)u18q4b67 (author)
  • Svensson, Anne-SophieKTH Royal Institute of Technology,KTH,Proteinvetenskap(Swepub:kth)u1t0mckt (author)
  • Jensen, GabriellaKTH Royal Institute of Technology,KTH,Proteinvetenskap(Swepub:kth)u1056u6d (author)
  • Enstedt, HenricKTH Royal Institute of Technology,KTH,Proteinteknologi(Swepub:kth)u1svlatx (author)
  • Afshari, DelaramKTH Royal Institute of Technology,KTH,Proteinteknologi(Swepub:kth)u18nn4qt (author)
  • Xu, Lan LanKTH Royal Institute of Technology,KTH,Proteinvetenskap(Swepub:kth)u1qu1kuf (author)
  • Zwahlen, MartinKTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1fcjpxe (author)
  • von Feilitzen, KalleKTH Royal Institute of Technology,KTH,Systembiologi,Proteinteknologi,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1dbmi38 (author)
  • Hanke, L.Karolinska Institutet (author)
  • Murrell, B.Karolinska Institutet (author)
  • McInerney, G.Karolinska Institutet (author)
  • Hedestam, G. B. K.Karolinska Institutet (author)
  • Lendel, ChristoferKTH Royal Institute of Technology,KTH,Tillämpad fysikalisk kemi(Swepub:kth)u1zepbos (author)
  • Roth, Robert G.AstraZeneca, Sweden(Swepub:lu)biok-rro (author)
  • Skoog, Ingmar,1954University of Gothenburg,Gothenburg University,Göteborgs universitet,Centrum för åldrande och hälsa (AgeCap),Centre for Ageing and Health (Agecap)(Swepub:gu)xskooi (author)
  • Svenungsson, E.Karolinska Institutet (author)
  • Olsson, T.Karolinska Institutet,Karolinska Institute (author)
  • Fogdell-Hahn, A.Karolinska Institutet,Karolinska Institute (author)
  • Lindroth, YlvaLund University,Lunds universitet,Avdelningen för medicinsk mikrobiologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Medical Microbiology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)med-yll (author)
  • Lundgren, MariaRegion Skåne(Swepub:lu)ma4744lu (author)
  • Maleki, K. T.Karolinska Institutet,Karolinska Institute,Public Health Agency of Sweden (author)
  • Lagerqvist, N.Public Health Agency of Sweden (author)
  • Klingstrom, J.Karolinska Institutet,Karolinska Institute,Public Health Agency of Sweden (author)
  • Rodrigues, R. D.Karolinska University Hospital (author)
  • Muschiol, S.Karolinska Institutet (author)
  • Bogdanovic, G. (author)
  • Sivertsson, ÅsaKTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Systembiologi(Swepub:kth)u195d5b0 (author)
  • Eklund, CarinaKarolinska Institutet(Swepub:lu)mikr-cek (author)
  • Lagheden, CamillaKarolinska Institutet(Swepub:lu)mikr-cla (author)
  • Dillner, JoakimKarolinska Institutet(Swepub:lu)mikr-jdi (author)
  • Sivertsson, A. (author)
  • Havervall, S.Karolinska Institutet (author)
  • Thalin, C.Karolinska Institutet (author)
  • Tegel, HannaKTH Royal Institute of Technology,KTH,Proteinvetenskap(Swepub:kth)u1nou8yx (author)
  • Pin, ElisaKTH Royal Institute of Technology,KTH,Affinitets-proteomik,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1pmjyje (author)
  • Månberg, Anna,1985-KTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik(Swepub:kth)u1g5roxd (author)
  • Hedhammar, My,Professor,1975-KTH Royal Institute of Technology,KTH,Centrum för Bioprocessteknik, CBioPT,Proteinteknologi,Science for Life Laboratory, SciLifeLab(Swepub:kth)u1o2n86z (author)
  • Nilsson, PeterKTH Royal Institute of Technology,KTH,Science for Life Laboratory, SciLifeLab,Affinitets-proteomik(Swepub:kth)u1ws88sk (author)
  • Arroyo Mühr, Laila SaraKarolinska Institute (author)
  • KTH Royal Institute of TechnologyAffinitets-proteomik (creator_code:org_t)

Related titles

  • In:Clinical & Translational Immunology: Wiley10:72050-0068

Internet link

Find in a library

To the university's database

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Close

Copy and save the link in order to return to this view