Sökning: WFRF:(Lerner Ulf)
> (2020-2024) >
Phytocystatin CsinC...
Phytocystatin CsinCPI-2 Reduces Osteoclastogenesis and Alveolar Bone Loss
-
Leguizamon, N. D. (författare)
-
de Molo, R. S. (författare)
-
Coletto-Nunes, G. (författare)
-
visa fler...
-
Nogueira, A. V. B. (författare)
-
Rocha, S. V. (författare)
-
Neo-Justino, D. M. (författare)
-
Soares-Costa, A. (författare)
-
Cerri, P. S. (författare)
-
- Lerner, Ulf H (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Centre for Bone and Arthritis Research,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
-
Souza, P. P. C. (författare)
-
Cirelli, J. A. (författare)
-
visa färre...
-
(creator_code:org_t)
- 2021-07-30
- 2022
- Engelska.
-
Ingår i: Journal of Dental Research. - : SAGE Publications. - 0022-0345 .- 1544-0591. ; 101:2, s. 216-225
- Relaterad länk:
-
https://gup.ub.gu.se...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Periodontal disease (PD) is a polymicrobial chronic inflammatory condition of the supporting tissues around the teeth, leading to the destruction of surrounding connective tissue. During the progression of PD, osteoclasts play a crucial role in the resorption of alveolar bone that eventually leads to the loss of teeth if the PD is left untreated. Therefore, the development of antiresorptive therapies targeting bone-resorbing cells will significantly benefit the treatment of PD. Here, we demonstrate the inhibitory effect of CsinCPI-2, a novel cysteine peptidase inhibitor from the orange tree, on periodontitis-induced inflammation, alveolar bone loss, and osteoclast differentiation. Using the ligature-induced periodontitis model in mice, we show that treatment with CsinCPI-2 (0.8 mu g/g of body weight) significantly reduced inflammatory cell infiltrate in the connective tissue and prevented the loss of alveolar bone mass (BV/TV) caused by PD, effects associated with diminished numbers of TRAP-positive multinucleated cells. Furthermore, CsinCPI-2 significantly downregulated the numbers of inflammatory cells expressing CD3, CD45, MAC387, and IL-1 beta. In vitro, CsinCPI-2 inhibited RANKL-induced TRAP+ multinucleated osteoclast formation in mouse bone marrow macrophage cultures in a concentration-dependent manner. This effect was not due to cytotoxicity, as demonstrated by the MTT assay. CsinCPI-2 inhibited RANKL-induced mRNA expression of Acp5, Calcr, and Ctsk, as well as the RANKL-induced upregulation of Nfatc1, a crucial transcription factor for osteoclast differentiation. Based on our findings, CsinCPI-2 prevents bone loss induced by PD by controlling the inflammatory process and acting directly on osteoclastogenesis, suggesting an interesting potential for CsinCPI-2 in the strategy for PD treatment.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Nyckelord
- periodontal diseases
- periodontitis
- bone resorption
- osteoclasts
- inflammation
- cystatins
- resorption in-vitro
- factor-kappa-b
- periodontal-disease
- cystatin-c
- sugarcane cystatin
- osteoimmunology
- osteonecrosis
- activation
- expression
- cytokines
- Dentistry
- Oral Surgery & Medicine
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Leguizamon, N. D ...
-
de Molo, R. S.
-
Coletto-Nunes, G ...
-
Nogueira, A. V. ...
-
Rocha, S. V.
-
Neo-Justino, D. ...
-
visa fler...
-
Soares-Costa, A.
-
Cerri, P. S.
-
Lerner, Ulf H
-
Souza, P. P. C.
-
Cirelli, J. A.
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Odontologi
- Artiklar i publikationen
-
Journal of Denta ...
- Av lärosätet
-
Göteborgs universitet