Search: WFRF:(Kübel Joachim 1988) >
The three-dimension...
The three-dimensional structure of Drosophila melanogaster (6-4) photolyase at room temperature
-
- Cellini, Andrea, 1991 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
- Wahlgren, Weixiao Yuan, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
- Henry, Léocadie (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
show more...
-
Pandey, S. (author)
-
- Ghosh, Swagatha, 1987 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
- Castillon, Leticia (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
- Claesson, Elin, 1989 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
Takala, H. (author)
-
- Kübel, Joachim, 1988 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
- Nimmrich, Amke, 1995 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
Kuznetsova, V. (author)
-
Nango, E. (author)
-
Iwata, S. (author)
-
Owada, S. (author)
-
Stojkovic, E. A. (author)
-
Schmidt, M. (author)
-
Ihalainen, J. A. (author)
-
- Westenhoff, Sebastian, 1978 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology
-
show less...
-
(creator_code:org_t)
- 2021-07-29
- 2021
- English.
-
In: Acta Crystallographica Section D-Structural Biology. - : International Union of Crystallography (IUCr). - 2059-7983. ; 77, s. 1001-1009
- Related links:
-
https://journals.iuc...
-
show more...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- (6-4) photolyases are flavoproteins that belong to the photolyase/cryptochrome family. Their function is to repair DNA lesions using visible light. Here, crystal structures of Drosophila melanogaster (6-4) photolyase [Dm(6-4)photolyase] at room and cryogenic temperatures are reported. The room-temperature structure was solved to 2.27 angstrom resolution and was obtained by serial femtosecond crystallography (SFX) using an X-ray free-electron laser. The crystallization and preparation conditions are also reported. The cryogenic structure was solved to 1.79 angstrom resolution using conventional X-ray crystallography. The structures agree with each other, indicating that the structural information obtained from crystallography at cryogenic temperature also applies at room temperature. Furthermore, UV-Vis absorption spectroscopy confirms that Dm(6-4)photolyase is photoactive in the crystals, giving a green light to time-resolved SFX studies on the protein, which can reveal the structural mechanism of the photoactivated protein in DNA repair.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- photolyases
- flavoproteins
- FAD
- serial crystallography
- room-temperature structure
- Drosophila melanogaster
- (6-4) photolyase
- serial femtosecond crystallography
- coli DNA photolyase
- x-ray-diffraction
- crystal-structure
- radical transfer
- repair activity
- mechanism
- cryptochrome
- photoactivation
- reconstitution
- Biochemistry & Molecular Biology
- Biophysics
- Crystallography
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Cellini, Andrea, ...
-
Wahlgren, Weixia ...
-
Henry, Léocadie
-
Pandey, S.
-
Ghosh, Swagatha, ...
-
Castillon, Letic ...
-
show more...
-
Claesson, Elin, ...
-
Takala, H.
-
Kübel, Joachim, ...
-
Nimmrich, Amke, ...
-
Kuznetsova, V.
-
Nango, E.
-
Iwata, S.
-
Owada, S.
-
Stojkovic, E. A.
-
Schmidt, M.
-
Ihalainen, J. A.
-
Westenhoff, Seba ...
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biophysics
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Biological Scien ...
-
and Biochemistry and ...
- Articles in the publication
-
Acta Crystallogr ...
- By the university
-
University of Gothenburg