Search: WFRF:(Ekström Karin 1978)
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Characterization of...
Characterization of surface markers on extracellular vesicles isolated from lymphatic exudate from patients with breast cancer
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- Ekström, Karin, 1977 (author)
- Gothenburg University,Göteborgs universitet,Wallenberglaboratoriet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Wallenberg Laboratory,Institute of Clinical Sciences, Department of Surgery
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- Crescitelli, Rossella, 1985 (author)
- Gothenburg University,Göteborgs universitet,Wallenberg Centre for Molecular and Translational Medicine,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery
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- Pétursson, Hafsteinn Ingi, 1977 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery
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Johansson, Junko, 1989 (author)
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- Lässer, Cecilia, 1981 (author)
- Gothenburg University,Göteborgs universitet,Krefting Research Centre
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- Olofsson Bagge, Roger, 1978 (author)
- Gothenburg University,Göteborgs universitet,Sahlgrenska Centrum för Cancerforskning (SCCR),Wallenberg Centre for Molecular and Translational Medicine,Sahlgrenska Center for Cancer Research (SCCR)
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(creator_code:org_t)
- 2022-01-10
- 2022
- English.
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In: Bmc Cancer. - : Springer Science and Business Media LLC. - 1471-2407. ; 22:1
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https://bmccancer.bi...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Background Breast cancer is the most common cancer, and the leading cause of cancer-related deaths, among females world-wide. Recent research suggests that extracellular vesicles (EVs) play a major role in the development of breast cancer metastasis. Axillary lymph node dissection (ALND) is a procedure in patients with known lymph node metastases, and after surgery large amounts of serous fluid are produced from the axilla. The overall aim was to isolate and characterize EVs from axillary serous fluid, and more specifically to determine if potential breast cancer biomarkers could be identified. Methods Lymphatic drain fluid was collected from 7 patients with breast cancer the day after ALND. EVs were isolated using size exclusion chromatography, quantified and detected by nanoparticle tracking analysis, electron microscopy, nano flow cytometry and western blot. The expression of 37 EV surface proteins was evaluated by flow cytometry using the MACSPlex Exosome kit. Results Lymphatic drainage exudate retrieved after surgery from all 7 patients contained EVs. The isolated EVs were positive for the typical EV markers CD9, CD63, CD81 and Flotillin-1 while albumin was absent, indicating low contamination from blood proteins. In total, 24 different EV surface proteins were detected. Eleven of those proteins were detected in all patients, including the common EV markers CD9, CD63 and CD81, cancer-related markers CD24, CD29, CD44 and CD146, platelet markers CD41b, CD42a and CD62p as well as HLA-DR/DP/DQ. Furthermore, CD29 and CD146 were enriched in Her2+ patients compared to patients with Her2- tumors. Conclusions Lymphatic drainage exudate retrieved from breast cancer patients after surgery contains EVs that can be isolated using SEC isolation. The EVs have several cancer-related markers including CD24, CD29, CD44 and CD146, proteins of potential interest as biomarkers as well as to increase the understanding of the mechanisms of cancer biology.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- Extracellular vesicles
- Exosomes
- Breast cancer
- Lymphatic drainage
- exudate
- Flow cytometry
- Multiplex phenotyping
- expression
- exosomes
- cells
- biomarkers
- cd44
- Oncology
Publication and Content Type
- ref (subject category)
- art (subject category)
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