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Conditioned serum i...
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Löfgren, MariaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health
(författare)
Conditioned serum in vitro treatment of chondrocyte pellets and osteoarthritic explants
- Artikel/kapitelEngelska2023
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
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LIBRIS-ID:oai:gup.ub.gu.se/317075
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https://gup.ub.gu.se/publication/317075URI
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https://doi.org/10.1111/evj.13582DOI
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https://res.slu.se/id/publ/117555URI
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Background: Autologous conditioned serum (ACS) is used to treat osteoarthritis in horses, although its effects are not fully investigated. Objectives: To investigate the effects of equine serum and conditioned serum on chondrocytes stimulated with interleukin (IL)-1 beta and cartilage explants with mild osteoarthritis. Study design: In vitro experimental study. Methods: The effect of three different serum preparations (unincubated control [PS], serum incubated 24 h [PS24h] and serum incubated 24 h in ACS containers [PCS]) pooled from lame horses were tested in two in vitro models. IL-1 beta and IL-1 receptor antagonist (IL-1Ra) concentrations were measured in all sera. In model 1, chondrocyte pellet cultures were stimulated with IL-1 beta prior to treatment with the serum preparations for 2 and 48 h. Microarray, polymerase chain reaction, and matrix metallopeptidase-13 analyses were performed. In model 2, cartilage explants from horses with structural osteoarthritis were treated with PS or PCS on days 0, 6 and 12, or left untreated, and evaluated at day 24 using the OARSI grading scale for histological evaluation of articular cartilage. Results: The IL-1Ra concentration in PS24h and PCS was significantly higher than in PS. In model 1, inflammation- and cartilage matrix degradation-related genes were upregulated after 48 h in all treatment groups versus untreated controls. Cartilage matrix molecules, aggrecan and collagens, were downregulated in PS24h- and PCS-treated pellets versus untreated controls. Growth factor signalling genes were upregulated-FGF7 in all treatment groups, BMP2 in PS24h-, and INHBA in PCS-treated-compared with untreated controls. In model 2, the OARSI score at day 24 was not significantly different between treatment groups. Main limitations: Results from in vitro models cannot be directly translated to in vivo situations. Conclusions: In vitro treatment with conditioned serum did not alleviate IL-1 beta-induced responses in chondrocyte pellets or lead to morphological improvement in osteoarthritic cartilage explants.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Ekman, StinaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health(Swepub:slu)47073
(författare)
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Ekholm, JosefineGothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine(Swepub:gu)xekbjo
(författare)
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Engström, MonaGothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine
(författare)
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Fjordbakk, C. T.
(författare)
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Svala, EmiliaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health(Swepub:slu)48811
(författare)
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Holm Forsström, KarinSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,University Animal Hospital(Swepub:slu)47899
(författare)
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Lindahl, Anders,1954Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine(Swepub:gu)xlandy
(författare)
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Skiöldebrand, EvaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Gothenburg University,Göteborgs universitet,Institutionen för biomedicin,Institute of Biomedicine,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health,Sahlgrenska University Hospital(Swepub:slu)50022
(författare)
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Sveriges lantbruksuniversitetInst för biomedicin och veterinär folkhälsovetenskap
(creator_code:org_t)
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Sveriges lantbruksuniversitet
Sammanhörande titlar
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Ingår i:Equine Veterinary Journal: Wiley55:2, s. 325-3350425-16442042-3306
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Löfgren, Maria
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Ekman, Stina
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Ekholm, Josefine
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Engström, Mona
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Fjordbakk, C. T.
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Svala, Emilia
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Holm Forsström, ...
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Lindahl, Anders, ...
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Skiöldebrand, Ev ...
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Equine Veterinar ...
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Göteborgs universitet
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Sveriges Lantbruksuniversitet