Expression level of Sec62 modulates membrane insertion of marginally hydrophobic segments

• In the endoplasmic reticulum (ER) membrane, transmembrane (TM) domain insertion occurs through the Sec61 channel with its auxiliary components, including Sec62. Sec62 interacts with the Sec61 channel and is located on the front side of the Sec61 lateral gate, an entry site for TM domains to the lipid bilayer. Overexpression of Sec62 led to a growth defect in yeast, and we investigated its effects on protein translocation and membrane insertion by pulse labeling of Sec62 client proteins. Our data show that the insertion efficiency of marginally hydrophobic TM segments is reduced upon Sec62 overexpression. This result suggests a potential regulatory role of Sec62 as a gatekeeper of the lateral gate, thereby modulating the insertion threshold of TM segments.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

Sec62

Overexpression

Membrane insertion

Marginally hydrophobic

Transmembrane

Biosynthesis

endoplasmic-reticulum

protein

yeast

translocation

integration

complex

Biochemistry & Molecular Biology

Biophysics

Publikations- och innehållstyp

ref (ämneskategori)

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