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Detection of SARS-C...
Detection of SARS-CoV-2 antibodies in serum and dried blood spot samples of vaccinated individuals using a sensitive homogeneous proximity extension assay.
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- Zhao, Hongxing (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Molekylära verktyg och funktionsgenomik,Unit Affin Prote Uppsala, Sci Life Lab, Stockholm, Sweden
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- Wang, Mengqi (author)
- Uppsala universitet,Molekylära verktyg och funktionsgenomik,Science for Life Laboratory, SciLifeLab
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- Muthelo, Phathutshedzo (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Molekylära verktyg och funktionsgenomik
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- Löf, Liza (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Molekylära verktyg och funktionsgenomik,Unit Affin Prote Uppsala, Sci Life Lab, Stockholm, Sweden
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- Sterky, Fredrik H (author)
- Gothenburg University,Göteborgs universitet,Wallenberg Centre for Molecular and Translational Medicine,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine,Department of Laboratory Medicine, University of Gothenburg, Sweden; Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Sweden; Department of Clinical Chemistry, Sahlgrenska University Hospital, Gothenburg, Sweden,Univ Gothenburg, Dept Lab Med, Gothenburg, Sweden.;Univ Gothenburg, Wallenberg Ctr Mol & Translat Med, Gothenburg, Sweden.;Sahlgrens Univ Hosp, Dept Clin Chem, Gothenburg, Sweden.
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- Gallini, Radiosa (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Unit Affin Prote Uppsala, Sci Life Lab, Stockholm, Sweden
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- Kumar, Nallani Vijay (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,Mammalian Protein Expression core facility, University of Gothenburg, Sweden,Univ Gothenburg, Mammalian Prot Express core facil, Gothenburg, Sweden.
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- Monsen, Tor J. (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Umeå Univ, Dept Clin Microbiol, Umeå, Sweden.
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- Nilsson, Kenneth, Docent, 1953- (author)
- Uppsala universitet,Klinisk mikrobiologi
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- Åberg, Mikael (author)
- Uppsala universitet,Klinisk mikrobiologi,Unit Affin Prote Uppsala, Sci Life Lab, Stockholm, Sweden
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- Kamali-Moghaddam, Masood (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Molekylära verktyg och funktionsgenomik
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- Mei, Ya-Fang (author)
- Umeå universitet,Institutionen för klinisk mikrobiologi,Umeå Univ, Dept Clin Microbiol, Umeå, Sweden.
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- Landegren, Ulf (author)
- Uppsala universitet,Science for Life Laboratory, SciLifeLab,Molekylära verktyg och funktionsgenomik
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(creator_code:org_t)
- Elsevier BV, 2022
- 2022
- English.
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In: New biotechnology. - : Elsevier BV. - 1871-6784 .- 1876-4347. ; 72, s. 139-148
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https://umu.diva-por... (primary) (Raw object)
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- A homogeneous PCR-based assay for sensitive and specific detection of antibodies in serum or dried blood spots (DBS) is presented and the method is used to monitor individuals infected with or vaccinated against SARS-CoV-2. Detection probes were prepared by conjugating the recombinant spike protein subunit 1 (S1), containing the receptor binding domain (RBD) of SARS-CoV-2, to each of a pair of specific oligonucleotides. The same was done for the nucleocapsid protein (NP). Upon incubation with serum or DBS samples, the bi- or multivalency of the antibodies (IgG, IgA or IgM) brings pairs of viral proteins with their conjugated oligonucleotides in proximity, allowing the antibodies to be detected by a modified proximity extension assay (PEA). Anti-S1 and anti-NP antibodies could be detected simultaneously from one incubation reaction. This Antibody PEA (AbPEA) test uses only 1µl of neat or up to 100,000-fold diluted serum or one ø1.2mm disc cut from a DBS. All 100 investigated sera and 21 DBS collected prior to the COVID-19 outbreak were negative, demonstrating a 100% specificity. The area under the curve, as evaluated by Receiver Operating Characteristic (ROC) analysis reached 0.998 (95%CI: 0.993-1) for samples taken from 11 days after symptoms onset. The kinetics of antibody responses were monitored after a first and second vaccination using serially collected DBS from 14 individuals. AbPEA offers highly specific and sensitive solution-phase antibody detection without requirement for secondary antibodies, no elution step when using DBS sample in a simple procedure that lends itself to multiplex survey of antibody responses.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Biomedicinsk laboratorievetenskap/teknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Biomedical Laboratory Science/Technology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Humans
- SARS-CoV-2
- COVID-19
- diagnosis
- Biological Assay
- Antibodies
- Kinetics
- Oligonucleotides
- Antibodies
- Viral
- Finger-prick dried blood spot
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Zhao, Hongxing
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Wang, Mengqi
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Muthelo, Phathut ...
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Löf, Liza
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Sterky, Fredrik ...
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Gallini, Radiosa
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Kumar, Nallani V ...
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Monsen, Tor J.
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Nilsson, Kenneth ...
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Åberg, Mikael
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Kamali-Moghaddam ...
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Mei, Ya-Fang
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Landegren, Ulf
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Medical Biotechn ...
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and Biomedical Labor ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Infectious Medic ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Immunology in th ...
- Articles in the publication
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New biotechnolog ...
- By the university
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University of Gothenburg
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Umeå University
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Uppsala University