The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay

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Sökning: AMNE:(LANTBRUKSVETENSKAPER Veterinärmedicin Patobiologi) > (2020-2024) > The expression of n...

Nystrom, S. (författare)

The expression of nerve growth factor in healthy and inflamed equine chondrocytes analysed by capillary western immunoassay

Artikel/kapitelEngelska2022

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Elsevier BV,2022

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LIBRIS-ID:oai:gup.ub.gu.se/322294
https://gup.ub.gu.se/publication/322294URI
https://doi.org/10.1016/j.rvsc.2022.08.015DOI
https://res.slu.se/id/publ/119810URI

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Språk:engelska

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Ämneskategori:art swepub-publicationtype

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Nerve Growth Factor (NGF) is a signalling molecule for pain and inflammation. NGF is increased in synovial fluid from osteoarthritic humans and animals, compared to healthy controls. Monoclonal antibody therapy directed against NGF has been approved to treat pain in osteoarthritic dogs but despite many years of trialling, therapy has not been approved for human use. One reason for this is that adverse reactions with rapidly progressing osteoarthritis has occurred in some individuals. More detailed knowledge of NGF expression in joints is needed. In this study, capillary-based Simple Western was used to analyse NGF in cultured equine chondrocytes. Chondrocytes were collected post mortem from three macroscopically healthy intercarpal joints and three intercarpal joints with mild osteoarthritic changes. The chondrocytes were expanded to passage one and seeded in chondrogenic medium to maintain the phenotype. On day four, cells were either stimulated with LPS or kept untreated in medium. All cells were harvested on day five. Wes analysis of lysates did not show mature NGF but two proforms, 40 and 45 kDa, were identified. Results were confirmed with western blot. The same proforms were expressed in chondrocytes from healthy and osteoarthritic joints. Acute inflammation induced by LPS stimulation did not change the forms of expressed NGF. Capillary Simple Western offers a sensitive and sample -sparing alternative to traditional western blot. However, confirmation of peaks is imperative in order to avoid misinterpretation of findings. In addition, in this case the method did not offer the possibility of quantification advertised by the manufacturers.

Ämnesord och genrebeteckningar

LANTBRUKSVETENSKAPER Veterinärmedicin hsv//swe
AGRICULTURAL SCIENCES Veterinary Science hsv//eng
LANTBRUKSVETENSKAPER Veterinärmedicin Patobiologi hsv//swe
AGRICULTURAL SCIENCES Veterinary Science Pathobiology hsv//eng
Equine
Chondrocyte
Osteoarthritis
Nerve growth factor
Simple western
synovial-fluid
factor ngf
precursor
beta
prongf
trka
Veterinary Sciences

Biuppslag (personer, institutioner, konferenser, titlar ...)

Kendall, AnnaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health(Swepub:slu)51201 (författare)
Adepu, S.Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health (författare)
Lindahl, Anders,1954Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för laboratoriemedicin,Department of Laboratory Medicine(Swepub:gu)xlandy (författare)
Skiöldebrand, EvaSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Inst för biomedicin och veterinär folkhälsovetenskap,Department of Biomedical Science and Veterinary Public Health(Swepub:slu)50022 (författare)
Sveriges lantbruksuniversitetInst för biomedicin och veterinär folkhälsovetenskap (creator_code:org_t)
Sveriges lantbruksuniversitet

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Ingår i:Research in Veterinary Science: Elsevier BV151, s. 156-1630034-52881532-2661

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Av författaren/redakt...: Nystrom, S.; Kendall, Anna; Adepu, S.; Lindahl, Anders, ...; Skiöldebrand, Ev ...

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LANTBRUKSVETENSKAPER: LANTBRUKSVETENSK ...; och Veterinärmedicin; och Patobiologi

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Av lärosätet: Göteborgs universitet; Sveriges Lantbruksuniversitet

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