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- Schneider, KaraGothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology (author)
Growth Rate Evaluation of the Budding Yeast Saccharomyces cerevisiae Cells Carrying Endogenously Expressed Fluorescent Protein Fusions
- Article/chapterEnglish2023
Publisher, publication year, extent ...
- 2022-09-16
- New York, NY :Springer US,2023
Numbers
- LIBRIS-ID:oai:gup.ub.gu.se/322558
- https://gup.ub.gu.se/publication/322558URI
- https://doi.org/10.1007/978-1-0716-2667-2_10DOI
Supplementary language notes
- Language:English
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- SwePubSwePub
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Notes
- Fluorescent proteins within fluorescent fusions have been reported to affect cellular growth fitness via altering native protein function and intracellular localization. Here we report in detail a procedure to analyze the growth characteristics of yeast cells expressing such fusions in comparison to unmodified parental strain. This approach can serve as an initial step in fluorescent protein characterization in vivo. © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Subject headings and genre
- NATURVETENSKAP Biologi Biokemi och molekylärbiologi hsv//swe
- NATURAL SCIENCES Biological Sciences Biochemistry and Molecular Biology hsv//eng
- Fluorescence
- Growth curve
- Protein fusion
- Yeast
- budding yeast
- growth rate
- in vivo study
- nonhuman
- protein analysis
- yeast cell
- genetics
- metabolism
- Saccharomyces cerevisiae
- coloring agent
- photoprotein
- Saccharomyces cerevisiae protein
- Coloring Agents
- Luminescent Proteins
- Saccharomyces cerevisiae Proteins
- Saccharomycetales
Added entries (persons, corporate bodies, meetings, titles ...)
- Reibenspies, Lucas E.Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology (author)
- Nyström, Thomas,1960Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology(Swepub:gu)xnysth (author)
- Shashkova, Sviatlana,1987Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Department of Mathematical Sciences,Institute of Biomedicine, Department of Microbiology and Immunology(Swepub:gu)xshasv (author)
- Göteborgs universitetInstitutionen för biomedicin, avdelningen för mikrobiologi och immunologi (creator_code:org_t)
Related titles
- In:Fluorezcent proteinsNew York, NY : Springer US1064-3745
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