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Sökning: id:"swepub:oai:gup.ub.gu.se/325151" > Prolonged Inflammat...

  • Dutta, Tanmoy,1998Gothenburg University,Göteborgs universitet,Institutionen för medicin,Institute of Medicine (författare)

Prolonged Inflammation and Infectious Changes in the Corneal Epithelium Are Associated with Persistent Epithelial Defect (PED)

  • Artikel/kapitelEngelska2023

Förlag, utgivningsår, omfång ...

  • 2023-02-06
  • MDPI AG,2023

Nummerbeteckningar

  • LIBRIS-ID:oai:gup.ub.gu.se/325151
  • https://gup.ub.gu.se/publication/325151URI
  • https://doi.org/10.3390/pathogens12020261DOI

Kompletterande språkuppgifter

  • Språk:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Purpose: Failure of rapid re-epithelialization within 10-14 days after corneal injury, even with standard supportive treatment, is referred to as persistent corneal epithelial (CE) defect (PED). Though an array of genes regulates reepithelization, their mechanisms are poorly understood. We sought to understand the network of genes driving the re-epithelialization in PED. Method: After obtaining informed consent, patients underwent an ophthalmic examination. Epithelial scrapes and tears samples of six PED patients and six individuals (control) undergoing photorefractive keratectomy (PRK) were collected. RNA isolation and quantification were performed using either the epithelial scrape taken from PED patients or from HCLE cells treated with control tears or tears of PED patients. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression of a few important genes in CE homeostasis, inflammation, and cell-cell communication, viz., Kruppel-like factor 4 (KLF4), GPX4, IL6, TNF alpha, STING, IL8, desmoglein, and E-cadherin, among others. Their expressions were normalized with their respective housekeeping genes and fold changes were recorded. KLF4 localization and MMPs activity was carried out via immunofluorescence and zymography, respectively. Results: KLF4, a transcription factor important for CE homeostasis, was upregulated in tears-treated HCLE cells and downregulated in PED patients compared to the healthy PRK group. Cell-cell communication genes were also upregulated in tears-treated cells, whereas they were downregulated in the PED tissue group. Genes involved in proinflammation (IL6, 282-fold; TNF alpha, 43-fold; IL8, 4.2-fold) were highly upregulated in both conditions. MMP9 activity increased upon tears treatment. Conclusions: This study suggests that tears create an acute proinflammatory milieu driving the PED disease pathology, whereas the PED patients scrapes are an indicator of the chronic stage of the disease. Interferons, pro-inflammatory genes, and their pathways are involved in PED, which can be a potential target for inducing epithelialization of the cornea.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Sangwan, J. (författare)
  • Mondal, M. (författare)
  • Vohra, M. (författare)
  • Nidhi, V. (författare)
  • Gour, A. (författare)
  • Kapur, N. (författare)
  • Gupta, N. (författare)
  • Bhowmick, T. (författare)
  • Chandru, A. (författare)
  • Mathur, U. (författare)
  • Sangwan, V. S. (författare)
  • Acharya, M. (författare)
  • Tiwari, A. (författare)
  • Göteborgs universitetInstitutionen för medicin (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Pathogens: MDPI AG12:22076-0817

Internetlänk

Hitta via bibliotek

  • Pathogens (Sök värdpublikationen i LIBRIS)

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