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Investigating New A...
Investigating New Applications of a Photoswitchable Fluorescent Norbornadiene as a Multifunctional Probe for Delineation of Amyloid Plaque Polymorphism.
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- Dreos, Ambra, 1987 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry,University of Gothenburg
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- Ge, Junyue, 1993 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry,University of Gothenburg
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- Najera, Francisco (author)
- Universidad De Malaga,University of Malaga
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- Tebikachew, Behabitu Ergette, 1985 (author)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Perez-Inestrosa, Ezequiel (author)
- Universidad De Malaga,University of Malaga
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- Moth-Poulsen, Kasper, 1978 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Institut de Ciència de Materials de Barcelona (ICMAB-CSIC),Institute of Material Science of Barcelona (ICMAB),Institucio Catalana de Recerca i Estudis Avancats (ICREA),Catalan Institution for Research and Advanced Studies (ICREA),Universitat Politecnica de Catalunya,Polytechnic University of Catalonia
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- Blennow, Kaj, 1958 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry,University of Gothenburg,Sahlgrenska universitetssjukhuset,Sahlgrenska University Hospital
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- Zetterberg, Henrik, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry,University College London (UCL),University of Gothenburg,Sahlgrenska universitetssjukhuset,Sahlgrenska University Hospital,University of Wisconsin Madison
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- Hanrieder, Jörg, 1980 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi,Institute of Neuroscience and Physiology, Department of Psychiatry and Neurochemistry,University College London (UCL),University of Gothenburg
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(creator_code:org_t)
- 2023-03-22
- 2023
- English.
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In: ACS sensors. - : American Chemical Society (ACS). - 2379-3694. ; 8:4, s. 1500-1509
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Abstract
Subject headings
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- Amyloid beta (Aβ) plaques are a major pathological hallmark of Alzheimer's disease (AD) and constitute of structurally heterogenic entities (polymorphs) that have been implicated in the phenotypic heterogeneity of AD pathology and pathogenesis. Understanding amyloid aggregation has been a critical limiting factor to gain understanding of AD pathogenesis, ultimately reflected in that the underlying mechanism remains elusive. We identified a fluorescent probe in the form of a turn-off photoswitchable norbornadiene derivative (NBD1) with several microenvironment-sensitive properties that make it relevant for applications within advanced fluorescence imaging, for example, multifunctional imaging. We explored the application of NBD1 for in situ delineation of structurally heterogenic Aβ plaques in transgenic AD mouse models. NBD1 plaque imaging shows characteristic broader emission bands in the periphery and more narrow emission bands in the dense cores of mature cored plaques. Further, we demonstrate in situ photoisomerization of NBD1 to quadricyclane and thermal recovery in single plaques, which is relevant for applications within both functional and super-resolution imaging. This is the first time a norbornadiene photoswitch has been used as a probe for fluorescence imaging of Aβ plaque pathology in situ and that its spectroscopic and switching properties have been studied within the specific environment of senile Aβ plaques. These findings open the way toward new applications of NBD-based photoswitchable fluorescent probes for super-resolution or dual-color imaging and multifunctional microscopy of amyloid plaque heterogeneity. This could allow to visualize Aβ plaques with resolution beyond the diffraction limit, label different plaque types, and gain insights into their physicochemical composition.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
Keyword
- Mice
- Animals
- Amyloid beta-Peptides
- chemistry
- Plaque
- Amyloid
- pathology
- Disease Models
- Animal
- Spectrometry
- Mass
- Matrix-Assisted Laser Desorption-Ionization
- methods
- Alzheimer Disease
- diagnostic imaging
- pathology
- Mice
- Transgenic
- Fluorescent Dyes
- amyloid beta (Aβ)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Dreos, Ambra, 19 ...
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Ge, Junyue, 1993
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Najera, Francisc ...
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Tebikachew, Beha ...
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Perez-Inestrosa, ...
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Moth-Poulsen, Ka ...
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show more...
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Blennow, Kaj, 19 ...
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Zetterberg, Henr ...
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Hanrieder, Jörg, ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Neurosciences
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Atom and Molecul ...
- Articles in the publication
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ACS sensors
- By the university
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University of Gothenburg
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Chalmers University of Technology