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  • Gruhl, T. (author)

Ultrafast structural changes direct the first molecular events of vision

  • Article/chapterEnglish2023

Publisher, publication year, extent ...

  • 2023-03-22
  • Springer Science and Business Media LLC,2023

Numbers

  • LIBRIS-ID:oai:gup.ub.gu.se/328122
  • https://gup.ub.gu.se/publication/328122URI
  • https://doi.org/10.1038/s41586-023-05863-6DOI

Supplementary language notes

  • Language:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Vision is initiated by the rhodopsin family of light-sensitive G protein-coupled receptors (GPCRs)(1). A photon is absorbed by the 11-cis retinal chromophore of rhodopsin, which isomerizes within 200 femtoseconds to the all-trans conformation(2), thereby initiating the cellular signal transduction processes that ultimately lead to vision. However, the intramolecular mechanism by which the photoactivated retinal induces the activation events inside rhodopsin remains experimentally unclear. Here we use ultrafast time-resolved crystallography at room temperature(3) to determine how an isomerized twisted all-trans retinal stores the photon energy that is required to initiate the protein conformational changes associated with the formation of the G protein-binding signalling state. The distorted retinal at a 1-ps time delay after photoactivation has pulled away from half of its numerous interactions with its binding pocket, and the excess of the photon energy is released through an anisotropic protein breathing motion in the direction of the extracellular space. Notably, the very early structural motions in the protein side chains of rhodopsin appear in regions that are involved in later stages of the conserved class A GPCR activation mechanism. Our study sheds light on the earliest stages of vision in vertebrates and points to fundamental aspects of the molecular mechanisms of agonist-mediated GPCR activation.

Subject headings and genre

Added entries (persons, corporate bodies, meetings, titles ...)

  • Weinert, T. (author)
  • Rodrigues, M. J. (author)
  • Milne, C. J. (author)
  • Ortolani, Giorgia,1985Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xortgi (author)
  • Nass, K. (author)
  • Nango, E. (author)
  • Sen, S. (author)
  • Johnson, P. J. M. (author)
  • Cirelli, C. (author)
  • Furrer, A. (author)
  • Mous, S. (author)
  • Skopintsev, P. (author)
  • James, D. (author)
  • Dworkowski, F. (author)
  • Båth, Petra,1988Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xbatpe (author)
  • Kekilli, D. (author)
  • Ozerov, D. (author)
  • Tanaka, R. (author)
  • Glover, H. (author)
  • Bacellar, C. (author)
  • Brunle, S. (author)
  • Casadei, C. M. (author)
  • Diethelm, A. D. (author)
  • Gashi, D. (author)
  • Gotthard, G. (author)
  • Guixa-Gonzalez, R. (author)
  • Joti, Y. (author)
  • Kabanova, V. (author)
  • Knopp, G. (author)
  • Lesca, E. (author)
  • Ma, P. K. Y. (author)
  • Martiel, I. (author)
  • Mnhle, J. (author)
  • Owada, S. (author)
  • Pamula, F. (author)
  • Sarabi, Daniel,1987Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xsarad (author)
  • Tejero, O. (author)
  • Tsai, C. J. (author)
  • Varma, N. (author)
  • Wach, A. (author)
  • Boutet, S. (author)
  • Tono, K. (author)
  • Nogly, P. (author)
  • Deupi, X. (author)
  • Iwata, S. (author)
  • Neutze, Richard,1969Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology(Swepub:gu)xneuri (author)
  • Standfuss, J. (author)
  • Schertler, G. (author)
  • Panneels, V. (author)
  • Göteborgs universitetInstitutionen för kemi och molekylärbiologi (creator_code:org_t)

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  • In:Nature: Springer Science and Business Media LLC615, s. 939-9440028-08361476-4687

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