Simultaneous inhibition of DNA-PK and Pol ϴ improves integration efficiency and precision of genome editing

Wimberger, Sandra,1987Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology (author)

Genome editing, specifically CRISPR/Cas9 technology, has revolutionized biomedical research and offers potential cures for genetic diseases. Despite rapid progress, low efficiency of targeted DNA integration and generation of unintended mutations represent major limitations for genome editing applications caused by the interplay with DNA double-strand break repair pathways. To address this, we conduct a large-scale compound library screen to identify targets for enhancing targeted genome insertions. Our study reveals DNA-dependent protein kinase (DNA-PK) as the most effective target to improve CRISPR/Cas9-mediated insertions, confirming previous findings. We extensively characterize AZD7648, a selective DNA-PK inhibitor, and find it to significantly enhance precise gene editing. We further improve integration efficiency and precision by inhibiting DNA polymerase theta (Pol ϴ). The combined treatment, named 2iHDR, boosts templated insertions to 80% efficiency with minimal unintended insertions and deletions. Notably, 2iHDR also reduces off-target effects of Cas9, greatly enhancing the fidelity and performance of CRISPR/Cas9 gene editing. Low efficiency of target DNA integration remains a challenge in genome engineering. Here the authors perform large-scale compound library and genetic screens to identify targets that enhance gene editing: they see that combined DNA-PK and Pol ϴ inhibition with potent compounds increases editing efficiency and precision.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Medicinsk genetik hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Medical Genetics hsv//eng
double-strand breaks
ligase-iii
targeted integration
protein-kinase
repair
ligation
crispr-cas9
discovery
platform
mouse
Science & Technology - Other Topics

Akrap, N. (author)
Firth, M. (author)
Brengdahl, J. (author)
Engberg, S. (author)
Schwinn, M. K. (author)
Slater, M. R. (author)
Lundin, A. (author)
Hsieh, P. P. (author)
Li, S. Y. (author)
Cerboni, S. (author)
Sumner, J. (author)
Bestas, B. (author)
Schiffthaler, B. (author)
Magnusson, B. (author)
Di Castro, S. (author)
Iyer, P. (author)
Bohlooly-Y, M. (author)
Machleidt, T. (author)
Rees, S. (author)
Engkvist, O. (author)
Norris, T. (author)
Cadogan, E. B. (author)
Forment, J. V. (author)
Svikovic, S. (author)
Akcakaya, P. (author)
Taheri-Ghahfarokhi, A. (author)
Maresca, M. (author)
Göteborgs universitetInstitutionen för kemi och molekylärbiologi (creator_code:org_t)

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Medical Genetics

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.