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Type IV Collagen in...
Type IV Collagen in Human Colorectal Liver Metastases—Cellular Origin and a Circulating Biomarker
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- Lindgren, Moa (author)
- Umeå universitet,Kirurgi
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- Rask, Gunilla (author)
- Umeå universitet,Patologi,Kirurgi
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- Jonsson, Josefin (author)
- Umeå universitet,Kirurgi
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- Berglund, Anette, 1960- (author)
- Umeå universitet,Kirurgi
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- Lundin, Christina, 1970- (author)
- Umeå universitet,Kirurgi
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- Jonsson, Pär (author)
- Umeå universitet,Kemiska institutionen
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- Ljuslinder, Ingrid, 1968- (author)
- Umeå universitet,Onkologi
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- Nyström, Hanna, 1980- (author)
- Umeå universitet,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Kirurgi
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(creator_code:org_t)
- 2022-07-13
- 2022
- English.
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In: Cancers. - : MDPI. - 2072-6694. ; 14:14
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https://doi.org/10.3...
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https://umu.diva-por... (primary) (Raw object)
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Abstract
Subject headings
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- Circulating type IV collagen (cCOL IV) is a potential biomarker for patients with colorectal liver metastases (CLM) who present with elevated levels of COL IV in both CLM tissue and circulation. This study aimed to establish the cellular origin of elevated levels of COL IV and analyze circulating COL IV in CLM patients. The cellular source was established through in situ hybridization, immunohistochemical staining, and morphological evaluation. Cellular expression in vitro was assessed by immunofluorescence. Tissue expression of COL IV-degrading matrix metalloproteinases (MMPs)-2, -7, -9, and -13 was studied with immunohistochemical staining. Plasma levels of COL IV in CLM patients and healthy controls were analyzed with ELISA. This study shows that cancer-associated fibroblasts (CAFs) express COL IV in the stroma of CLM and that COL IV is expressed in vitro by fibroblasts but not by tumor cells. MMP-2, -7, -9, and -13 are expressed in CLM tissue, mainly by hepatocytes and immune cells, and circulating COL IV is significantly elevated in CLM patients compared with healthy controls. Our study shows that stromal cells, not tumor cells, produce COL IV in CLM, and that circulating COL IV is elevated in patients with CLM.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Keyword
- CAF
- circulating biomarkers
- COL IV
- colorectal cancer
- fibroblasts
- liver metastases
- MMP
- tumor stroma
- type IV collagen
Publication and Content Type
- ref (subject category)
- art (subject category)
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