Stimulation of skeletal stem cells in the growth plate promotes linear bone growth

Trompet, Dana,1993Gothenburg University,Göteborgs universitet,Centre for Bone and Arthritis Research,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition (författare)

Recently, skeletal stem cells were shown to be present in the epiphyseal growth plate (epiphyseal skeletal stem cells, epSSCs), but their function in connection with linear bone growth remains unknown. Here, we explore the possibility that modulating the number of epSSCs can correct differences in leg length. First, we examined regulation of the number and activity of epSSCs by Hedgehog (Hh) signaling. Both systemic activation of Hh pathway with Smoothened agonist (SAG) and genetic activation of Hh pathway by Patched1 (Ptch1) ablation in Pthrp-creER Ptch1(fl/fl )tdTomato mice promoted proliferation of epSSCs and clonal enlargement. Transient intra-articular administration of SAG also elevated the number of epSSCs. When SAG-containing beads were implanted into the femoral secondary ossification center of 1 leg of rats, this leg was significantly longer 1 month later than the contralateral leg implanted with vehicle-containing beads, an effect that was even more pronounced 2 and 6 months after implantation. We conclude that Hh signaling This opens therapeutic possibilities for the treatment of differences in leg length.

Kurenkova, Anastasiia D. (författare)
Zhou, Baoyi (författare)
Li, Lei,1985Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Centre for Bone and Arthritis Research,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition(Swepub:gu)xlilew (författare)
Dregval, Ostap,1992Gothenburg University,Göteborgs universitet,Institutionen för medicin,Centre for Bone and Arthritis Research,Institute of Medicine(Swepub:gu)xdreos (författare)
Usanova, Anna P. (författare)
Chu, Tsz Long,1988Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Centre for Bone and Arthritis Research,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition(Swepub:gu)xchuts (författare)
Are, AlexandraKarolinska Institutet (författare)
Nedorubov, Andrei A. (författare)
Kasper, MariaKarolinska Institutet (författare)
Chagin, Andrei S.,1976Gothenburg University,Göteborgs universitet,Centre for Bone and Arthritis Research,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition(Swepub:gu)xchaga (författare)
Göteborgs universitetCentre for Bone and Arthritis Research (creator_code:org_t)

Av författaren/redakt...: Trompet, Dana, 1 ...; Kurenkova, Anast ...; Zhou, Baoyi; Li, Lei, 1985; Dregval, Ostap, ...; Usanova, Anna P.; visa fler...; Chu, Tsz Long, 1 ...; Are, Alexandra; Nedorubov, Andre ...; Kasper, Maria; Chagin, Andrei S ...; visa färre...

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