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Tracking early mamm...
Tracking early mammalian organogenesis - prediction and validation of differentiation trajectories at whole organism scale
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Imaz-Rosshandler, Ivan (författare)
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Rode, Christina (författare)
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- Guibentif, Carolina (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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Harland, Luke T. G. (författare)
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Ton, Mai-Linh N. (författare)
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Dhapola, Parashar (författare)
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Keitley, Daniel (författare)
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Argelaguet, Ricard (författare)
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Calero-Nieto, Fernando J. (författare)
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Nichols, Jennifer (författare)
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Marioni, John C. (författare)
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de Bruijn, Marella F. T. R. (författare)
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Gottgens, Berthold (författare)
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(creator_code:org_t)
- 2024
- 2024
- Engelska.
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Ingår i: DEVELOPMENT. - 0950-1991 .- 1477-9129. ; 151:3
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Early organogenesis represents a key step in animal development, during which pluripotent cells diversify to initiate organ formation. Here, we sampled 300,000 single-cell transcriptomes from mouse embryos between E8.5 and E9.5 in 6-h intervals and combined this new dataset with our previous atlas (E6.5-E8.5) to produce a densely sampled timecourse of >400,000 cells from early gastrulation to organogenesis. Computational lineage reconstruction identified complex waves of blood and endothelial development, including a new programme for somite-derived endothelium. We also dissected the E7.5 primitive streak into four adjacent regions, performed scRNA-seq and predicted cell fates computationally. Finally, we defined developmental state/ fate relationships by combining orthotopic grafting, microscopic analysis and scRNA-seq to transcriptionally determine cell fates of grafted primitive streak regions after 24 h of in vitro embryo culture. Experimentally determined fate outcomes were in good agreement with computationally predicted fates, demonstrating how classical grafting experiments can be revisited to establish high-resolution cell state/fate relationships. Such interdisciplinary approaches will benefit future studies in developmental biology and guide the in vitro production of cells for organ regeneration and repair.
Ämnesord
- NATURVETENSKAP -- Biologi -- Utvecklingsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Developmental Biology (hsv//eng)
Nyckelord
- Cell fate and differentiation
- Haematopoiesis
- Mouse development
- Single-cell transcriptomics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Imaz-Rosshandler ...
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Rode, Christina
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Guibentif, Carol ...
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Harland, Luke T. ...
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Ton, Mai-Linh N.
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Dhapola, Parasha ...
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visa fler...
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Keitley, Daniel
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Argelaguet, Rica ...
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Calero-Nieto, Fe ...
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Nichols, Jennife ...
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Marioni, John C.
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de Bruijn, Marel ...
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Gottgens, Bertho ...
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visa färre...
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Göteborgs universitet