Bispectral fluorescence imaging combined with texture analysis and linear discrimination for correlation with histopathologic extent of basal cell carcinoma

• Fluorescence imaging has been shown to be a potential complement to visual inspection for demarcation of basal cell carcinoma (BCC), which is the most common type of skin cancer. Earlier studies have shown promising results when combining autofluorescence with protoporphyrin IX (Pp IX) fluorescence, induced by application of delta-5-aminolaevulinic acid (ALA). In this work, we have tried to further improve the ability of this technique to discriminate between areas of tumor and normal skin by implementing texture analysis and Fisher linear discrimination (FLD) on bispectral fluorescence data of BCCs located on the face. Classification maps of the lesions have been obtained from histopathologic mapping of the excised tumors. The contrast feature obtained from co-occurrence matrices was found to provide useful information, particularly for the ALA-induced Pp IX fluorescence data. Moreover, the neighborhood average features of both autofluorescence and Pp IX fluorescence were preferentially included in the analysis. The algorithm was trained by using a training set of images with good agreement with histopathology, which improved the discriminability of the validation set. In addition, cross validation of the training set showed good discriminability. Our results imply that FLD and texture analysis are preferential for correlation between bispectral fluorescence images and the histopathologic extension of the tumors.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)

Nyckelord

Adult

Aged

Aged

80 and over

*Algorithms

*Artificial Intelligence

Carcinoma

Basal Cell/classification/*pathology

Computer Simulation

Data Interpretation

Statistical

Discriminant Analysis

Female

Humans

Image Interpretation

Computer-Assisted/*methods

Linear Models

Male

Microscopy

Fluorescence/*methods

Middle Aged

Models

Biological

Neoplasm Invasiveness

Neoplasm Staging/methods

Reproducibility of Results

Sensitivity and Specificity

Severity of Illness Index

Skin Neoplasms/classification/*pathology

Spectrometry

Fluorescence/methods

Statistics

Adult

Publikations- och innehållstyp

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