Newcastle disease virus neuraminidase primes neutrophils for stimulation by galectin-3 and formyl-Met-Leu-Phe

• Human neutrophils are activated by the beta-galactoside-binding lectin galectin-3, provided that the cells are primed by in vivo extravasation or by in vitro preactivation with, for example, LPS. Removal of terminal sialic acid can change neutrophil functionality and responsiveness due to exposure of underlying glycoconjugate receptors or change in surface charge. Here, we investigated whether such alteration of the cell surface carbohydrate composition can alter the responsiveness of the cells to galectin-3. Neutrophils were treated with neuraminidases (NA) of different origins: Clostridium perfringens (CP), Salmonella typhimurium, Vibrio cholerae, and Newcastle disease virus (NDV). In the presence of NDV-NA, but no other NA, the otherwise non-responding neutrophils responded readily to galectin-3 by activation of the NADPH-oxidase. The galectin-3 priming effect was inhibited by the sialidase inhibitor 2,3-dehydro-2-deoxy-N-acetyl-neuraminic acid. Earlier studies have shown that priming of the neutrophil response to galectin-3 with, for example, LPS is paralleled by degranulation of intracellular vesicles and granules and upregulation of potential galectin-3 receptors. Also, NDV-NA (but not CP-NA) treatment induced degranulation, shown as an upregulation of complement receptor 3. Since not only the galectin response but also the response to the chemoattractant fMLF was primed, NDV-NA appears to induce a general priming phenomenon, possibly due to receptor upregulation by degranulation.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Microbiology in the medical area (hsv//eng)

MEDICIN OCH HÄLSOVETENSKAP  -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Basic Medicine -- Immunology in the medical area (hsv//eng)

Nyckelord

Cell Degranulation/drug effects/immunology/physiology

Chemotaxis

Leukocyte/immunology

Enzyme Inhibitors/pharmacology

Galectin 3/*immunology/metabolism

Humans

Membrane Glycoproteins/drug effects/immunology

N-Acetylneuraminic Acid/metabolism

N-Formylmethionine Leucyl-Phenylalanine/*immunology/metabolism

NADPH Oxidase/metabolism

Neuraminidase/antagonists & inhibitors/*immunology/metabolism/pharmacology

Neutrophil Activation/*immunology

Neutrophils/drug effects/*immunology/virology

Newcastle disease virus/*enzymology/immunology

Receptors

Cell Surface/drug effects/immunology

Receptors

Complement/drug effects/immunology

Respiratory Burst/drug effects/immunology/physiology

Secretory Vesicles/drug effects/metabolism

Temperature

Up-Regulation/drug effects/immunology

Viral Proteins/immunology

respiratory burst

inflammation

galectins

neutrophils

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)