Search: id:"swepub:oai:gup.ub.gu.se/41509" >
Impact of tobacco s...
-
Andersson, AndersGothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi,Institute of Internal Medicine, Dept of Respiratory Medicine/Allergology
(author)
Impact of tobacco smoke on interleukin-16 protein in human airways, lymphoid tissue and T lymphocytes
- Article/chapterEnglish2004
Publisher, publication year, extent ...
-
2004-08-18
-
Oxford University Press (OUP),2004
Numbers
-
LIBRIS-ID:oai:gup.ub.gu.se/41509
-
https://gup.ub.gu.se/publication/41509URI
-
https://doi.org/10.1111/j.1365-2249.2004.02580.xDOI
-
https://lup.lub.lu.se/record/898470URI
Supplementary language notes
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
CD4(+) and CD8(+) lymphocytes are mobilized in severe chronic obstructive pulmonary disease (COPD) and the CD8(+) cytokine interleukin (IL)-16 is believed to be important in regulating the recruitment and activity of CD4(+) lymphocytes. In the current study, we examined whether tobacco smoke exerts an impact not only on IL-16 in the lower airways but also in CD4(+) or CD8(+) lymphocytes or in lymphoid tissue. The concentration of IL-16 protein was measured by enzyme-linked immunosorbent assay (ELISA) in concentrated bronchoalveolar lavage fluid (BALF) collected from 33 smokers with chronic bronchitis (CB), eight asymptomatic smokers (AS) and seven healthy never-smokers (NS). The concentrations of IL-16 and soluble IL-2 receptor alpha (sIL-2Ralpha) protein were also measured in conditioned medium from human blood CD4(+) and CD8(+) lymphocytes stimulated with tobacco smoke extract (TSE) in vitro. IL-16 mRNA was assessed in vitro as well, using reverse transcription-polymerase chain reaction (RT-PCR). Finally, the intracellular immunoreactivity for IL-16 protein (IL-16IR) was assessed in six matched pairs of palatine tonsils from smokers and non-smokers. BALF IL-16 was higher in CB and AS than in NS. TSE substantially increased the concentration of IL-16 but not sIL-2Ralpha in conditioned medium from CD4(+) and CD8(+) lymphocytes. There was no corresponding effect on IL-16 mRNA. IL-16IR in tonsils was lower in smokers than in non-smokers. The current findings demonstrate that tobacco smoke exerts a wide impact on the CD8(+) cytokine IL-16, in the airway lumen, in blood CD4(+) and CD8(+) lymphocytes and in lymphoid tissue. The effect on IL-16 release may be selective for preformed IL-16 in CD4(+) lymphocytes. New clinical studies are required to evaluate whether tobacco smoke mobilizes T lymphocytes via IL-16 in the lower airways and whether this mechanism can be targeted in COPD.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Qvarfordt, Ingemar,1954Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi,Institute of Internal Medicine, Dept of Respiratory Medicine/Allergology(Swepub:gu)xqvain
(author)
-
Laan, Martti,1971Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi,Institute of Internal Medicine, Dept of Respiratory Medicine/Allergology
(author)
-
Sjöstrand, Margareta,1947Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi,Institute of Internal Medicine, Dept of Respiratory Medicine/Allergology(Swepub:gu)xsjosm
(author)
-
Malmhäll, Carina,1959Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi,Institute of Internal Medicine, Dept of Respiratory Medicine/Allergology(Swepub:gu)xmalmc
(author)
-
Riise, Gerdt C.,1956Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi,Institute of Internal Medicine, Dept of Respiratory Medicine/Allergology(Swepub:gu)xriige
(author)
-
Cardell, Lars-OlafLund University,Lunds universitet,Klinisk och experimentell allergiforskning,Laryngoesofagologi, allergi och livskvalitet,Forskargrupper vid Lunds universitet,Clinical and Experimental Allergy Research,Laryngoesophagology, Allergy and Life Quality,Lund University Research Groups(Swepub:lu)oron-lca
(author)
-
Lindén, Anders,1961Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi,Institute of Internal Medicine, Dept of Respiratory Medicine/Allergology(Swepub:gu)xlandd
(author)
-
Göteborgs universitetInstitutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi
(creator_code:org_t)
Related titles
-
In:Clinical and experimental immunology: Oxford University Press (OUP)138:1, s. 75-820009-91041365-2249
Internet link
Find in a library
To the university's database
- By the author/editor
-
Andersson, Ander ...
-
Qvarfordt, Ingem ...
-
Laan, Martti, 19 ...
-
Sjöstrand, Marga ...
-
Malmhäll, Carina ...
-
Riise, Gerdt C., ...
-
show more...
-
Cardell, Lars-Ol ...
-
Lindén, Anders, ...
-
show less...
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Clinical Medicin ...
-
and Respiratory Medi ...
-
- MEDICAL AND HEALTH SCIENCES
-
MEDICAL AND HEAL ...
-
and Basic Medicine
-
and Immunology in th ...
- Articles in the publication
-
Clinical and exp ...
- By the university
-
University of Gothenburg
-
Lund University