Using NMR metabolomics to identify responses of an environmental estrogen in blood plasma of fish

Samuelsson, Linda,1973Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology (author)

Nuclear magnetic resonance (NMR) based metabolomics in combination with multivariate data analysis may become valuable tools to study environmental effects of pharmaceuticals and other chemicals in aquatic organisms. To explore the usefulness of this approach in fish, we have used H-1 NMR metabolomics to compare blood plasma and plasma lipid extracts from rainbow trout exposed to the synthetic contraceptive estrogen ethinylestradiol (EE2) with plasma from control fish. The plasma metabolite profile was affected in fish exposed to 10 ng/L but not 0.87 ng/L of EE2, which was in agreement with an induced vitellogenin synthesis in the high dose group only, as measured by ELISA. The main affected metabolites were vitellogenin, alanine, phospholipids and cholesterol. The responses identified by this discovery-driven method could be put in context with previous knowledge of the effects of estrogens on fish. This adds confidence to the approach of using NMR metabolomics to identify environmental effects of pharmaceuticals and other contaminants. (c) 2006 Elsevier B.V. All rights reserved.

NATURVETENSKAP Kemi Analytisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
NATURVETENSKAP Geovetenskap och miljövetenskap Miljövetenskap hsv//swe
NATURAL SCIENCES Earth and Related Environmental Sciences Environmental Sciences hsv//eng
NATURVETENSKAP Biologi Mikrobiologi hsv//swe
NATURAL SCIENCES Biological Sciences Microbiology hsv//eng
metabolomics
NMR
rainbow trout
ethinylestradiol
vitellogenin
blood plasmaAnimals
Enzyme-Linked Immunosorbent Assay
veterinary
Estrogens
blood
toxicity
Ethinyl Estradiol
blood
toxicity
Female
Male
Multivariate Analysis
Nuclear Magnetic Resonance
Biomolecular
methods
Oncorhynchus mykiss
blood
Vitellogenins
biosynthesis
blood
Water Pollutants
Chemical
blood
toxicity

Förlin, Lars,1950Gothenburg University,Göteborgs universitet,Zoologiska institutionen,Department of Zoology(Swepub:gu)xfolar (author)
Karlsson, B Göran,1962Gothenburg University,Göteborgs universitet,Svenskt NMR-centrum vid Göteborgs universitet,Swedish NMR Centre at Göteborg University(Swepub:gu)xkargo (author)
Adolfsson-Erici, Margareta (author)
Larsson, D. G. Joakim,1969Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology(Swepub:gu)xlarjo (author)
Göteborgs universitetInstitutionen för neurovetenskap och fysiologi, sektionen för fysiologi (creator_code:org_t)

In:Aquatic Toxicology (Amsterdam, Netherlands): Elsevier BV78:4, s. 341-3490166-445X

By the author/editor: Samuelsson, Lind ...; Förlin, Lars, 19 ...; Karlsson, B Göra ...; Adolfsson-Erici, ...; Larsson, D. G. J ...

About the subject

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Analytical Chemi ...

NATURAL SCIENCES: NATURAL SCIENCES; and Earth and Relate ...; and Environmental Sc ...

NATURAL SCIENCES: NATURAL SCIENCES; and Biological Scien ...; and Microbiology

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.