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Leukotriene and pro...
Leukotriene and prostanoid pathway enzymes in bronchial biopsies of seasonal allergic asthmatics
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Seymour, M. L. (author)
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- Rak, Sabina, 1945 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
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- Åberg, Daniel, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin,Institute of Internal Medicine
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- Riise, Gerdt C., 1956 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för lungmedicin och allergologi,Institute of Internal Medicine, Dept of Respiratory Medicine/Allergology
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Penrose, J. F. (author)
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Kanaoka, Y. (author)
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Austen, K. F. (author)
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Holgate, S. T. (author)
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Sampson, A. P. (author)
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(creator_code:org_t)
- 2001
- 2001
- English.
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In: Am J Respir Crit Care Med. - 1073-449X. ; 164:11, s. 2051-6
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
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- Cysteinyl-leukotrienes and prostaglandin D2 generated by the 5-lipoxygenase (5-LO) and cyclooxygenase (COX) pathways, respectively, cause bronchoconstriction, leukocyte recruitment, and bronchial hyperresponsiveness in asthma. We characterized the cellular expression of 5-LO and COX enzymes using immunohistochemistry on bronchial biopsies from 12 allergic asthmatic patients before and during seasonal exposure to birch pollen. Bronchial responsiveness (p = 0.004) and symptoms (p < 0.005) increased and peak expiratory flow (PEF; p < or = 0.02) decreased in the pollen season. In-season biopsies had 2-fold more cells immunostaining for 5-LO (p = 0.02), 5-LO-activating protein (FLAP; p = 0.04), and leukotriene (LT)A4 hydrolase (p = 0.05), and 4-fold more for the terminal enzyme for cysteinyl-leukotriene synthesis, LTC4 synthase (p = 0.02). Immunostaining for COX-1, COX-2, and PGD2 synthase was unchanged. Increased staining for LTC4 synthase was due to increased eosinophils (p = 0.035) and an increased proportion of eosinophils expressing the enzyme (p = 0.047). Macrophages also increased (p = 0.019), but mast cells and T-lymphocyte subsets were unchanged. Inverse correlations between PEF and 5-LO(+) cell counts link increased expression of 5-LO pathway enzymes in eosinophils and macrophages within the bronchial mucosa to deterioration of lung function during seasonal allergen exposure.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)
Keyword
- Adult
- Air Pollution/adverse effects/analysis
- Arachidonate 5-Lipoxygenase/*analysis/immunology/*metabolism
- Asthma/etiology/*metabolism/*pathology/physiopathology
- Biopsy
- Bronchial Hyperreactivity/etiology/*metabolism/*pathology/physiopathology
- Eosinophils/immunology
- Forced Expiratory Volume
- Humans
- Hypersensitivity/etiology/*metabolism/*pathology/physiopathology
- Immunohistochemistry
- Leukocyte Count
- Leukotrienes/*analysis/immunology/*metabolism
- Macrophages/immunology
- Mast Cells/immunology
- Peak Expiratory Flow Rate
- Pollen/*adverse effects
- Prostaglandin-Endoperoxide Synthases/*analysis/immunology/*metabolism
- Prostaglandins/*analysis/immunology/*metabolism
- *Seasons
- Severity of Illness Index
- Sweden
- T-Lymphocytes/immunology
- Trees
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Seymour, M. L.
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Rak, Sabina, 194 ...
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Åberg, Daniel, 1 ...
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Riise, Gerdt C., ...
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Penrose, J. F.
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Kanaoka, Y.
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show more...
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Austen, K. F.
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Holgate, S. T.
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Sampson, A. P.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Dermatology and ...
- Articles in the publication
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Am J Respir Crit ...
- By the university
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University of Gothenburg